Identification of Fas (APO-1/CD95) and p53 gene mutations in non-small cell lung cancer

  • Authors:
    • L. Boldrini
    • P. Faviana
    • S. Gisfredi
    • D. Di Quirico
    • M. Lucchi
    • A. Mussi
    • C. A. Angeletti
    • F. Baldinotti
    • A. Fogli
    • P. Simi
    • F. Basolo
    • F. Pingitore
    • G. Fontanini
  • View Affiliations

  • Published online on: January 1, 2002     https://doi.org/10.3892/ijo.20.1.155
  • Pages: 155-159
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Abstract

Fas (APO-1/CD95) is a broadly expressed death receptor involved in a series of physiological and pathological apoptotic processes. One of the possible mechanisms for resistance to apoptosis signaling in the immune system as well as in the pathogenesis of non-lymphoid malignancies is the presence of Fas mutations within the entire gene. We investigated, in 79 non-small cell lung cancer (NSCLC) samples, the promoter and the entire coding region of the Fas gene by polymerase chain reaction, single strand conformation polymorphism and DNA sequencing in order to detect putative alterations. Sixteen of 79 tumor samples (20.2%) were found to have Fas alterations, either in promoter or exon region. Since the loss of Fas apoptotic function might be linked to p53 alterations, which are often involved in the development of NSCLC, we analyzed p53 status in 40 of the 79 NSCLC samples. p53 mutations were found to be more frequently present than Fas gene alterations (25 out of 40 cases, 62.5%). These data increase the knowledge regarding mutations of apoptosis-genes involved in the pathogenesis of NSCLC, and give benefits for the clinical management of this type of tumor.

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January 2002
Volume 20 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Boldrini, L., Faviana, P., Gisfredi, S., Di Quirico, D., Lucchi, M., Mussi, A. ... Fontanini, G. (2002). Identification of Fas (APO-1/CD95) and p53 gene mutations in non-small cell lung cancer. International Journal of Oncology, 20, 155-159. https://doi.org/10.3892/ijo.20.1.155
MLA
Boldrini, L., Faviana, P., Gisfredi, S., Di Quirico, D., Lucchi, M., Mussi, A., Angeletti, C. A., Baldinotti, F., Fogli, A., Simi, P., Basolo, F., Pingitore, F., Fontanini, G."Identification of Fas (APO-1/CD95) and p53 gene mutations in non-small cell lung cancer". International Journal of Oncology 20.1 (2002): 155-159.
Chicago
Boldrini, L., Faviana, P., Gisfredi, S., Di Quirico, D., Lucchi, M., Mussi, A., Angeletti, C. A., Baldinotti, F., Fogli, A., Simi, P., Basolo, F., Pingitore, F., Fontanini, G."Identification of Fas (APO-1/CD95) and p53 gene mutations in non-small cell lung cancer". International Journal of Oncology 20, no. 1 (2002): 155-159. https://doi.org/10.3892/ijo.20.1.155