IL-12 and IL-15 induce activation of nuclear PLCβ in human natural killer cells

  • Authors:
    • Cristina Ponti
    • Mirella Falconi
    • Anna Maria Billi
    • Irene Faenza
    • Sergio Castorina
    • Luigi Caimi
    • Antonio Cacchioli
    • Lucio Cocco
    • Marco Vitale
  • View Affiliations

  • Published online on: January 1, 2002     https://doi.org/10.3892/ijo.20.1.149
  • Pages: 149-153
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Abstract

Nuclear phosphoinositide breakdown mediated by nuclear PLCβ phosphorylation is a downstream effect of IL-2 stimulation in primary human NK cells that is involved in the proliferative response to IL-2. Here we investigated whether the nuclear phosphoinositide turnover in NK cells is a response confined to IL-2, or rather represents a more general mechanism of nuclear signalling linked to the proliferative response of NK cells to activatory cytokines. We therefore focused on IL-12 and IL-15-induced nuclear events in primary human NK cells. Our results show that IL-12 and IL-15 activate nuclear PLCβ1 in NK cells, with delayed kinetics as compared to IL-2. The nuclear PLC activation induced by the cytokines could be blocked by the MEK-1 inhibitor PD 98059, suggesting its dependence upon MAPKinase activation. Our conclusion is that the three cytokines that activate NK cells and that bind partially similar (IL-2, IL-15) or different surface receptors (IL-12), all induce activation of PLCβ1 in the nucleus of NK cells via MAPKinase. Thus, the activation of nuclear PLCβ1 appears as a physiologically relevant general mechanism of response to cytokine stimulation in human natural killer cells.

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January 2002
Volume 20 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ponti, C., Falconi, M., Billi, A.M., Faenza, I., Castorina, S., Caimi, L. ... Vitale, M. (2002). IL-12 and IL-15 induce activation of nuclear PLCβ in human natural killer cells. International Journal of Oncology, 20, 149-153. https://doi.org/10.3892/ijo.20.1.149
MLA
Ponti, C., Falconi, M., Billi, A. M., Faenza, I., Castorina, S., Caimi, L., Cacchioli, A., Cocco, L., Vitale, M."IL-12 and IL-15 induce activation of nuclear PLCβ in human natural killer cells". International Journal of Oncology 20.1 (2002): 149-153.
Chicago
Ponti, C., Falconi, M., Billi, A. M., Faenza, I., Castorina, S., Caimi, L., Cacchioli, A., Cocco, L., Vitale, M."IL-12 and IL-15 induce activation of nuclear PLCβ in human natural killer cells". International Journal of Oncology 20, no. 1 (2002): 149-153. https://doi.org/10.3892/ijo.20.1.149