Enhanced radiation killing by 5-fluorouracil of biliary tract cancer cell lines.

  • Authors:
    • Y Moon
    • T Todoroki
    • T Ohno
    • K Fukao
    • J B Little
  • View Affiliations

  • Published online on: May 1, 2000     https://doi.org/10.3892/ijo.16.5.987
  • Pages: 987-1081
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Abstract

Despite the frequent clinical use of 5-fluorouracil (5-FU) in combination with radiotherapy for patients with biliary tract cancers, data remain scarce concerning specifically the influence of 5-FU on the sensitivity of these cancer cells to radiation. The present study was carried out to evaluate the effects of concomitant treatment with 5-FU on radiation-induced cell killing in two established human biliary tract cancer cell lines (Mz-ChA-2 and SK-ChA-1 cells). These lines were chosen as we have previously shown that SK-ChA-1 cells are significantly more resistant to both radiation and 5-FU than Mz-ChA-2 cells. Clonogenic survival was employed as the end-point for cell killing. Administration of 5-FU at LD50 doses to each cell line significantly enhanced radiation-induced cell killing. The enhancement ratio (ER) was obtained by dividing the radiation dose required to decrease the cell survival fraction to 37% (D0) by the dose to decrease cell survival to the same level when the cells were also treated with 5-FU. The ER in each of the cell lines was greater when they were incubated with 5-FU after radiation rather than prior to radiation. Longer exposure times with 5-FU resulted in enhanced radiation killing. The ER was significantly higher in the radioresistant cell line than in the radiosensitive line. These findings suggest that therapy with radiation and 5-FU may be of value as components of multidisciplinary treatment for biliary tract cancer. Protracted low dose exposure to 5-FU may prove to be most efficacious in enhancing the effects of radiation therapy.

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May 2000
Volume 16 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Moon, Y., Todoroki, T., Ohno, T., Fukao, K., & Little, J. (2000). Enhanced radiation killing by 5-fluorouracil of biliary tract cancer cell lines.. International Journal of Oncology, 16, 987-1081. https://doi.org/10.3892/ijo.16.5.987
MLA
Moon, Y., Todoroki, T., Ohno, T., Fukao, K., Little, J."Enhanced radiation killing by 5-fluorouracil of biliary tract cancer cell lines.". International Journal of Oncology 16.5 (2000): 987-1081.
Chicago
Moon, Y., Todoroki, T., Ohno, T., Fukao, K., Little, J."Enhanced radiation killing by 5-fluorouracil of biliary tract cancer cell lines.". International Journal of Oncology 16, no. 5 (2000): 987-1081. https://doi.org/10.3892/ijo.16.5.987