Patched 2, located in 1p32-34, is not mutated in high stage neuroblastoma tumors.

  • Authors:
    • A Jögi
    • F Abel
    • R M Sjöberg
    • R Toftgård
    • P G Zaphiropoulos
    • S Påhlman
    • T Martinsson
    • H Axelson
  • View Affiliations

  • Published online on: May 1, 2000     https://doi.org/10.3892/ijo.16.5.943
  • Pages: 943-952
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Neuroblastoma is a childhood malignancy originating from cells of the sympathetic nervous system, exhibiting a marked diversity in outcome, with spontaneous regression at one end of the spectrum and severe disease and death at the other end. Features associated with frequent recurrence, a poor prognosis, and high tumor stage are loss of heterozygosity in the distal region of chromosome 1p and amplification of the N-myc gene. Patched 2 is a novel homologue to the tumor suppressor gene Patched 1, and has been mapped to 1p32-34, a part of chromosome 1 frequently deleted in high stage neuroblastoma tumors. RT-PCR analysis of 9 neuroblastoma cell lines showed expression of both Patched 1 and 2. We analyzed 14, mainly high stage, neuroblastoma tumors for mutations in the Patched 2 gene with denaturing HPLC using the Wave DNA fragment analysis system. In four tumor samples variations were detected within the coding sequence, and two of them gave rise to amino-acid substitutions. These variations were, however, also detected in normal DNA from the respective patients. We conclude that Patched 2 is expressed, but not frequently mutated, in high stage neuroblastomas and is therefore not likely to be involved in the genesis of this tumor.

Related Articles

Journal Cover

May 2000
Volume 16 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Jögi, A., Abel, F., Sjöberg, R., Toftgård, R., Zaphiropoulos, P., Påhlman, S. ... Axelson, H. (2000). Patched 2, located in 1p32-34, is not mutated in high stage neuroblastoma tumors.. International Journal of Oncology, 16, 943-952. https://doi.org/10.3892/ijo.16.5.943
MLA
Jögi, A., Abel, F., Sjöberg, R., Toftgård, R., Zaphiropoulos, P., Påhlman, S., Martinsson, T., Axelson, H."Patched 2, located in 1p32-34, is not mutated in high stage neuroblastoma tumors.". International Journal of Oncology 16.5 (2000): 943-952.
Chicago
Jögi, A., Abel, F., Sjöberg, R., Toftgård, R., Zaphiropoulos, P., Påhlman, S., Martinsson, T., Axelson, H."Patched 2, located in 1p32-34, is not mutated in high stage neuroblastoma tumors.". International Journal of Oncology 16, no. 5 (2000): 943-952. https://doi.org/10.3892/ijo.16.5.943