Establishment of a visible peritoneal micrometastatic model from a gastric adenocarcinoma cell line by green fluorescent protein.

  • Authors:
    • K Kaneko
    • M Yano
    • T Tsujinaka
    • S Morita
    • M Taniguchi
    • Y Fujiwara
    • Y Doki
    • M Inoue
    • H Shiozaki
    • M Monden
  • View Affiliations

  • Published online on: May 1, 2000     https://doi.org/10.3892/ijo.16.5.893
  • Pages: 893-901
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Abstract

To establish a visible peritoneal micrometastatic model, an enhanced green fluorescent protein (EGFP) expressing plasmid vector was transfected into the gastric cancer cell line, MKN-45. Highly expressing EGFP cells were injected into the peritoneal cavity of nude mice. Then, the peritoneum and abdominal organs were harvested and observed on days 1, 4 and 7. Fluorescence stereomicroscopy identified peritoneal micrometastases that had been undetected by stereomicroscopy. Micrometastases as small as a single cell are detectable in this model. This peritoneal micrometastatic model should be a useful tool for research on metastasis of gastric cancer.

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May 2000
Volume 16 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kaneko, K., Yano, M., Tsujinaka, T., Morita, S., Taniguchi, M., Fujiwara, Y. ... Monden, M. (2000). Establishment of a visible peritoneal micrometastatic model from a gastric adenocarcinoma cell line by green fluorescent protein.. International Journal of Oncology, 16, 893-901. https://doi.org/10.3892/ijo.16.5.893
MLA
Kaneko, K., Yano, M., Tsujinaka, T., Morita, S., Taniguchi, M., Fujiwara, Y., Doki, Y., Inoue, M., Shiozaki, H., Monden, M."Establishment of a visible peritoneal micrometastatic model from a gastric adenocarcinoma cell line by green fluorescent protein.". International Journal of Oncology 16.5 (2000): 893-901.
Chicago
Kaneko, K., Yano, M., Tsujinaka, T., Morita, S., Taniguchi, M., Fujiwara, Y., Doki, Y., Inoue, M., Shiozaki, H., Monden, M."Establishment of a visible peritoneal micrometastatic model from a gastric adenocarcinoma cell line by green fluorescent protein.". International Journal of Oncology 16, no. 5 (2000): 893-901. https://doi.org/10.3892/ijo.16.5.893