Identification and characterization of amino acid starvation-induced CD24 gene in MCF-7 human breast cancer cells.

  • Authors:
    • W Liu
    • J V Vadgama
  • View Affiliations

  • Published online on: May 1, 2000     https://doi.org/10.3892/ijo.16.5.1049
  • Pages: 1049-1103
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Abstract

Amino acid starvation is a pathophysiological condition that results in protein deprivation due to cancer cachexia. Using the method of differential display of reverse transcription PCR (DDRT-PCR), we isolated a cDNA fragment in MCF-7 human breast cancer cells in response to amino acid starvation, which was identical with human CD24 gene. Northern blot results showed that CD24 mRNA in MCF-7 cells was constitutively expressed and significantly upregulated upon amino acid starvation. This stimulation was time-dependent and the maximal response was at 24 h. The expression of the amino acid starvation-induced CD24 mRNA decreased when starved cells were returned to a medium supplemented with amino acids. This repressive response was also time-dependent. Amino acid starvation-induced CD24 mRNA expression in MCF-7 cells was completely blocked by actinomycin D, which suggested that the regulation of CD24 mRNA by amino acid availability occurred at transcriptional level. When amino acid-starved cells were refed with amino acids for 8 h, the expression of CD24 mRNA declined to the basal levels confirming that CD24 mRNA expression could be stimulated by amino acid starvation. Interestingly, CD24 mRNA was poorly detected in MCF-10 cells, a benign human breast epithelial cell line. In conclusion, CD24 mRNA expression in MCF-7 cells was upregulated upon amino acid starvation. This amino acid starvation-induced upregulation of CD24 mRNA occurred at transcriptional level. The regulation of CD24 mRNA in MCF-7 cells by amino acid availability may play an important role in the progression and metastasis of human breast cancer.

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May 2000
Volume 16 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Liu, W., & Liu, W. (2000). Identification and characterization of amino acid starvation-induced CD24 gene in MCF-7 human breast cancer cells.. International Journal of Oncology, 16, 1049-1103. https://doi.org/10.3892/ijo.16.5.1049
MLA
Liu, W., Vadgama, J."Identification and characterization of amino acid starvation-induced CD24 gene in MCF-7 human breast cancer cells.". International Journal of Oncology 16.5 (2000): 1049-1103.
Chicago
Liu, W., Vadgama, J."Identification and characterization of amino acid starvation-induced CD24 gene in MCF-7 human breast cancer cells.". International Journal of Oncology 16, no. 5 (2000): 1049-1103. https://doi.org/10.3892/ijo.16.5.1049