Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers.

  • Authors:
    • Y Fujiwara
    • Y Sugita
    • S Nakamori
    • A Miyamoto
    • K Shiozaki
    • H Nagano
    • M Sakon
    • M Monden
  • View Affiliations

  • Published online on: April 1, 2000     https://doi.org/10.3892/ijo.16.4.799
  • Pages: 799-1603
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Abstract

We assessed Stanniocalcin-1 (STC-1) mRNA expression in human normal tissues, various types of human cancer cell lines, and cancer tissues obtained during surgery. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay developed to detect STC-1 mRNA, the transcripts were detected in 20 out of 21 cancer cell lines and in all tumor tissues from various types of cancer. Semi-quantitative analyses with multiplex RT-PCR showed that STC-1 mRNA tended to be enhanced in cancer tissues of hepatocellular carcinoma and colorectal cancer compared to background cancer-free tissues. Analysis of blood samples obtained from 11 patients with hepatocellular carcinoma before, after and during hepatectomy showed STC-1 mRNA expression in 8 out of 11 patients at least one time. Normal donor blood samples (n=31) were all-negative for STC-1 mRNA expression. Our results indicate that STC-1 mRNA might be a useful molecular marker for detection of tumor cells in blood from patients with various types of malignancies.

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April 2000
Volume 16 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Fujiwara, Y., Sugita, Y., Nakamori, S., Miyamoto, A., Shiozaki, K., Nagano, H. ... Monden, M. (2000). Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers.. International Journal of Oncology, 16, 799-1603. https://doi.org/10.3892/ijo.16.4.799
MLA
Fujiwara, Y., Sugita, Y., Nakamori, S., Miyamoto, A., Shiozaki, K., Nagano, H., Sakon, M., Monden, M."Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers.". International Journal of Oncology 16.4 (2000): 799-1603.
Chicago
Fujiwara, Y., Sugita, Y., Nakamori, S., Miyamoto, A., Shiozaki, K., Nagano, H., Sakon, M., Monden, M."Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers.". International Journal of Oncology 16, no. 4 (2000): 799-1603. https://doi.org/10.3892/ijo.16.4.799