Expression of the OGG1-type 1a (nuclear form) protein in cancerous and non-cancerous human cells.

  • Authors:
    • K Shinmura
    • T Kohno
    • M Takeuchi-Sasaki
    • M Maeda
    • T Segawa
    • T Kamo
    • H Sugimura
    • J Yokota
  • View Affiliations

  • Published online on: April 1, 2000     https://doi.org/10.3892/ijo.16.4.701
  • Pages: 701-708
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The human OGG1 gene encodes 8-hydroxyguanine DNA glycosylase. By RT-PCR analysis, five novel type 1 transcripts, in addition to eight known types (OGG1-types 1a to 1c and 2a to 2e), were identified. Among them, only the type 1a isoform contains both a nuclear localization signal and the entire DNA binding motif, suggesting the involvement of type 1a in chromosomal DNA repair. By Western blot analysis using a monoclonal antibody prepared by immunizing the whole type 1a protein, a 39 kDa type 1a protein was detected in lung cancer cell lines and peripheral lymphocytes. The type 1a protein was expressed at a similar level, irrespective of its polymorphic types characterized by distinct repair activity. By an immunocytochemical study, the majority of type 1a protein was localized in the nucleus. These results indicate that OGG1-type 1a protein is involved in the repair of 8-hydroxyguanine in chromosomal double-stranded DNA and constitutively expressed in cancerous and non-cancerous human cells.

Related Articles

Journal Cover

April 2000
Volume 16 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Shinmura, K., Kohno, T., Takeuchi-Sasaki, M., Maeda, M., Segawa, T., Kamo, T. ... Yokota, J. (2000). Expression of the OGG1-type 1a (nuclear form) protein in cancerous and non-cancerous human cells.. International Journal of Oncology, 16, 701-708. https://doi.org/10.3892/ijo.16.4.701
MLA
Shinmura, K., Kohno, T., Takeuchi-Sasaki, M., Maeda, M., Segawa, T., Kamo, T., Sugimura, H., Yokota, J."Expression of the OGG1-type 1a (nuclear form) protein in cancerous and non-cancerous human cells.". International Journal of Oncology 16.4 (2000): 701-708.
Chicago
Shinmura, K., Kohno, T., Takeuchi-Sasaki, M., Maeda, M., Segawa, T., Kamo, T., Sugimura, H., Yokota, J."Expression of the OGG1-type 1a (nuclear form) protein in cancerous and non-cancerous human cells.". International Journal of Oncology 16, no. 4 (2000): 701-708. https://doi.org/10.3892/ijo.16.4.701