Detection of telomerase activity in human carcinomas using a trap-ELISA method: correlation with hTR and hTERT expression.

  • Authors:
    • R González-Quevedo
    • C de Juan
    • M J Massa
    • A Sánchez-Pernaute
    • A Torres
    • J L Balibrea
    • M Benito
    • P Iniesta
  • View Affiliations

  • Published online on: March 1, 2000     https://doi.org/10.3892/ijo.16.3.623
  • Pages: 623-631
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Abstract

We have evaluated telomerase activity in a tumour population of 65 human cancers by using a TRAP-based method, in which detection is performed by an enzyme immunoassay (ELISA). We have corroborated that sensitivity and specificity of this new procedure can be considered similar to that of classical TRAP method, having the advantage of a rapid and reproducible analysis of large pools of samples. Thus, telomerase activity was detected in 83% of the tumours included in our population. Moreover, we found a significant association between enzyme activity and both hTR and hTERT expression (P=0.004 and P=0.04, respectively).

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March 2000
Volume 16 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
González-Quevedo, R., de Juan, C., Massa, M., Sánchez-Pernaute, A., Torres, A., Balibrea, J. ... Iniesta, P. (2000). Detection of telomerase activity in human carcinomas using a trap-ELISA method: correlation with hTR and hTERT expression.. International Journal of Oncology, 16, 623-631. https://doi.org/10.3892/ijo.16.3.623
MLA
González-Quevedo, R., de Juan, C., Massa, M., Sánchez-Pernaute, A., Torres, A., Balibrea, J., Benito, M., Iniesta, P."Detection of telomerase activity in human carcinomas using a trap-ELISA method: correlation with hTR and hTERT expression.". International Journal of Oncology 16.3 (2000): 623-631.
Chicago
González-Quevedo, R., de Juan, C., Massa, M., Sánchez-Pernaute, A., Torres, A., Balibrea, J., Benito, M., Iniesta, P."Detection of telomerase activity in human carcinomas using a trap-ELISA method: correlation with hTR and hTERT expression.". International Journal of Oncology 16, no. 3 (2000): 623-631. https://doi.org/10.3892/ijo.16.3.623