The p16INK4alpha/p19ARF gene mutations are infrequent and are mutually exclusive to p53 mutations in Indian oral squamous cell carcinomas.

  • Authors:
    • K Kannan
    • A K Munirajan
    • J Krishnamurthy
    • V Bhuvarahamurthy
    • B K Mohanprasad
    • K H Panishankar
    • N Tsuchida
    • G Shanmugam
  • View Affiliations

  • Published online on: March 1, 2000     https://doi.org/10.3892/ijo.16.3.585
  • Pages: 585-675
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Abstract

Eighty-seven untreated primary oral squamous cell carcinomas (SCCs) associated with betel quid and tobacco chewing from Indian patients were analysed for the presence of mutations in the commonly shared exon 2 of p16INK4alpha/p19ARF genes. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing analysis were used to detect mutations. SSCP analysis indicated that only 9% (8/87) of the tumours had mutation in p16INK4alpha/p19ARF genes. Seventy-two tumours studied here were previously analysed for p53 mutations and 21% (15/72) of them were found to have mutations in p53 gene. Only one tumour was found to have mutation at both p53 and p16INK4alpha/p19ARF genes. Thus, the mutation rates observed were 21% for p53, 9% for p16INK4alpha/p19ARF, and 1% for both. Sequencing analysis revealed two types of mutations; i) G to C (GCAG to CCAG) transversion type mutation at intron 1-exon 2 splice junction and ii) another C to T transition type mutation resulting in CGA to TGA changing arginine to a termination codon at p16INK4alpha gene codon 80 and the same mutation will alter codon 94 of p19ARF gene from CCG to CTG (proline to leucine). These results suggest that p16INK4alpha/p19ARF mutations are less frequent than p53 mutations in Indian oral SCCs. The p53 and p16INK4alpha/p19ARF mutational events are independent and are mutually exclusive suggesting that mutational inactivation of either p53 or p16INK4alpha/p19ARF may alleviate the need for the inactivation of the other gene.

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March 2000
Volume 16 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kannan, K., Munirajan, A., Krishnamurthy, J., Bhuvarahamurthy, V., Mohanprasad, B., Panishankar, K. ... Shanmugam, G. (2000). The p16INK4alpha/p19ARF gene mutations are infrequent and are mutually exclusive to p53 mutations in Indian oral squamous cell carcinomas.. International Journal of Oncology, 16, 585-675. https://doi.org/10.3892/ijo.16.3.585
MLA
Kannan, K., Munirajan, A., Krishnamurthy, J., Bhuvarahamurthy, V., Mohanprasad, B., Panishankar, K., Tsuchida, N., Shanmugam, G."The p16INK4alpha/p19ARF gene mutations are infrequent and are mutually exclusive to p53 mutations in Indian oral squamous cell carcinomas.". International Journal of Oncology 16.3 (2000): 585-675.
Chicago
Kannan, K., Munirajan, A., Krishnamurthy, J., Bhuvarahamurthy, V., Mohanprasad, B., Panishankar, K., Tsuchida, N., Shanmugam, G."The p16INK4alpha/p19ARF gene mutations are infrequent and are mutually exclusive to p53 mutations in Indian oral squamous cell carcinomas.". International Journal of Oncology 16, no. 3 (2000): 585-675. https://doi.org/10.3892/ijo.16.3.585