Autocrine secreted insulin-like growth factor-I stimulates MAP kinase-dependent mitogenic effects in human primitive neuroectodermal tumor/medulloblastoma.

  • Authors:
    • R Patti
    • C D Reddy
    • B Geoerger
    • M A Grotzer
    • M Raghunath
    • L N Sutton
    • P C Phillips
  • View Affiliations

  • Published online on: March 1, 2000     https://doi.org/10.3892/ijo.16.3.577
  • Pages: 577-661
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Abstract

Primitive neuroectodermal tumors/medulloblastoma (PNET/MB) have similarities to neuroectodermal progenitor cells of the developing CNS. Since insulin-like growth factor I (IGF-I) exerts pleiotrophic effects on cells in the developing CNS, we evaluated the production, mitogenic effects and signaling pathways of IGF-I in PNET/MB cells and found that IGF-I is an autocrine growth factor in human PNET/MB cell lines tested. Stimulation of DAOY cells by IGF-I led to phosphorylation of its cognate receptor (IGF-IR) and resulted in cell proliferation. These effects of IGF-I were suppressed by IGF-IR blocking antibodies and by PD 98059, MAP kinase pathway inhibitor. The results demonstrate the existence of an autocrine IGF-I/IGF-IR loop and indicate that IGF-I promotes proliferation via MAP kinase pathway.

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March 2000
Volume 16 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Patti, R., Reddy, C., Geoerger, B., Grotzer, M., Raghunath, M., Sutton, L., & Phillips, P. (2000). Autocrine secreted insulin-like growth factor-I stimulates MAP kinase-dependent mitogenic effects in human primitive neuroectodermal tumor/medulloblastoma.. International Journal of Oncology, 16, 577-661. https://doi.org/10.3892/ijo.16.3.577
MLA
Patti, R., Reddy, C., Geoerger, B., Grotzer, M., Raghunath, M., Sutton, L., Phillips, P."Autocrine secreted insulin-like growth factor-I stimulates MAP kinase-dependent mitogenic effects in human primitive neuroectodermal tumor/medulloblastoma.". International Journal of Oncology 16.3 (2000): 577-661.
Chicago
Patti, R., Reddy, C., Geoerger, B., Grotzer, M., Raghunath, M., Sutton, L., Phillips, P."Autocrine secreted insulin-like growth factor-I stimulates MAP kinase-dependent mitogenic effects in human primitive neuroectodermal tumor/medulloblastoma.". International Journal of Oncology 16, no. 3 (2000): 577-661. https://doi.org/10.3892/ijo.16.3.577