Infrequent widespread microsatellite instability in hepatocellular carcinomas.

  • Authors:
    • H Yamamoto
    • F Itoh
    • H Fukushima
    • H Kaneto
    • S Sasaki
    • T Ohmura
    • T Satoh
    • Y Karino
    • T Endo
    • J Toyota
    • K Imai
  • View Affiliations

  • Published online on: March 1, 2000     https://doi.org/10.3892/ijo.16.3.543
  • Pages: 543-550
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Widespread or high-frequency microsatellite instability (MSI) due to the defective DNA mismatch repair (MMR) occurs in the majority of hereditary non-polyposis colorectal cancer and a subset of sporadic malignant tumors. The incidence of MSI and underlying DNA MMR defects have been well characterized in gastrointestinal carcinogenesis, but not in hepatocarcinogenesis. To address the issue, we analyzed 55 Japanese hepatocellular carcinomas using several indicators of DNA MMR defects, such as microsatellite analysis, loss of heterozygosity (LOH) and mutation analysis of MMR genes, methylation of hMLH1 promoter, and frameshift mutations of mononucleotide repeat sequences within possible target genes. Mutation of beta2-microglobulin gene, which is presumably involved in MSI-positive tumor cell escape from immune surveillance was also examined. Some of these analyses were also carried out in 9 human liver cancer cell lines. None of the 3 quasi-monomorphic mononucleotide markers sensitive for MSI, BAT26, BAT25, and BAT34C4 presented shortened unstable alleles in any of the carcinoma, cirrhosis, chronic hepatitis tissues, or cell lines. LOH at MMR genes was infrequent (4.4 approximately 7.1%), and no mutations were detected. Neither hMLH1 hypermethylation nor frameshift mutation in the target genes was detected. No mutations were found in beta2-microglobulin. Widespread MSI due to the defective DNA MMR appears to play little if any part in Japanese hepatocarcinogenesis.

Related Articles

Journal Cover

March 2000
Volume 16 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yamamoto, H., Itoh, F., Fukushima, H., Kaneto, H., Sasaki, S., Ohmura, T. ... Imai, K. (2000). Infrequent widespread microsatellite instability in hepatocellular carcinomas.. International Journal of Oncology, 16, 543-550. https://doi.org/10.3892/ijo.16.3.543
MLA
Yamamoto, H., Itoh, F., Fukushima, H., Kaneto, H., Sasaki, S., Ohmura, T., Satoh, T., Karino, Y., Endo, T., Toyota, J., Imai, K."Infrequent widespread microsatellite instability in hepatocellular carcinomas.". International Journal of Oncology 16.3 (2000): 543-550.
Chicago
Yamamoto, H., Itoh, F., Fukushima, H., Kaneto, H., Sasaki, S., Ohmura, T., Satoh, T., Karino, Y., Endo, T., Toyota, J., Imai, K."Infrequent widespread microsatellite instability in hepatocellular carcinomas.". International Journal of Oncology 16, no. 3 (2000): 543-550. https://doi.org/10.3892/ijo.16.3.543