Sampling technique influences the detection of K-ras mutations in normal appearing mucosa of colorectal cancer patients.

  • Authors:
    • C C Schimanski
    • C Sutter
    • U Linnemann
    • M R Berger
  • View Affiliations

  • Published online on: August 1, 1999     https://doi.org/10.3892/ijo.15.2.391
  • Pages: 391-399
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Abstract

Based on three colorectal cancer cell lines with specified K-ras status, a sensitive PCR-RFLP assay was established detecting one K-ras mutant among 106 wild-type cells. Using this assay for tissues of 124 colorectal cancer patients, 59 tumor (46%) and 11 mucosa samples (9%) were found to harbor a K-ras mutation. When using the same scalpel for collecting tumor and mucosa tissues (group A), 18% of the patients had a matching K-ras mutation in both tissues, but this coincidence was seen in 3% of patients only, when separate scalpels were used (group B). Thus we conclude that the sampling technique used for collecting specimens is a major contributor to the detection of K-ras mutations in normal appearing mucosa when a highly sensitive detection technique is used.

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August 1999
Volume 15 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Schimanski, C., Sutter, C., Linnemann, U., & Berger, M. (1999). Sampling technique influences the detection of K-ras mutations in normal appearing mucosa of colorectal cancer patients.. International Journal of Oncology, 15, 391-399. https://doi.org/10.3892/ijo.15.2.391
MLA
Schimanski, C., Sutter, C., Linnemann, U., Berger, M."Sampling technique influences the detection of K-ras mutations in normal appearing mucosa of colorectal cancer patients.". International Journal of Oncology 15.2 (1999): 391-399.
Chicago
Schimanski, C., Sutter, C., Linnemann, U., Berger, M."Sampling technique influences the detection of K-ras mutations in normal appearing mucosa of colorectal cancer patients.". International Journal of Oncology 15, no. 2 (1999): 391-399. https://doi.org/10.3892/ijo.15.2.391