Lack of mutations in DNA polymerase beta of estradiol-induced hamster kidney tumors: sequence of hamster DNA polymerase beta cDNA.

  • Authors:
    • L Y Kong
    • A V Hodgson
    • I N Springer
    • J G Liehr
  • View Affiliations

  • Published online on: August 1, 1999     https://doi.org/10.3892/ijo.15.2.353
  • Pages: 353-362
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We examined the effects of estradiol (E2), the natural estrogenic hormone, on the structure and expression of DNA polymerase beta (DNA pol beta), a DNA repair gene, from E2-induced primary kidney tumors of twelve Syrian hamsters, their metastases, and from kidney tissues surrounding the tumors. We sequenced the coding region of the hamster DNA pol beta and found it to differ from that of the human by 11%. No mutations were detected in the entire coding region including the catalytic domain of the DNA pol beta from E2-induced primary kidney tumors, their metastases, or from kidney tissues surrounding the tumors. The expression of the DNA pol beta mRNA was also not significantly altered in E2-induced kidney tumors or in kidney tissues surrounding the tumors compared to that of control kidney tissues. These results suggest that mutations in the DNA pol beta gene may not be involved in the induction or malignant progression of hamster kidney tumors induced by E2. The nucleotide sequence of the hamster DNA pol beta described here will be useful for the study of the structure and expression of this gene.

Related Articles

Journal Cover

August 1999
Volume 15 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kong, L., Hodgson, A., Springer, I., & Liehr, J. (1999). Lack of mutations in DNA polymerase beta of estradiol-induced hamster kidney tumors: sequence of hamster DNA polymerase beta cDNA.. International Journal of Oncology, 15, 353-362. https://doi.org/10.3892/ijo.15.2.353
MLA
Kong, L., Hodgson, A., Springer, I., Liehr, J."Lack of mutations in DNA polymerase beta of estradiol-induced hamster kidney tumors: sequence of hamster DNA polymerase beta cDNA.". International Journal of Oncology 15.2 (1999): 353-362.
Chicago
Kong, L., Hodgson, A., Springer, I., Liehr, J."Lack of mutations in DNA polymerase beta of estradiol-induced hamster kidney tumors: sequence of hamster DNA polymerase beta cDNA.". International Journal of Oncology 15, no. 2 (1999): 353-362. https://doi.org/10.3892/ijo.15.2.353