The association between a mutated ras gene and cyclooxygenase-2 expression in human breast cancer cell lines.

  • Authors:
    • E M Gilhooly
    • D P Rose
  • View Affiliations

  • Published online on: August 1, 1999     https://doi.org/10.3892/ijo.15.2.267
  • Pages: 267-337
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Abstract

Cyclooxygenase (COX) is rate-limiting for arachidonic acid metabolism to the prostanoid family of eicosanoids. Some human breast cancers, notably those which are estrogen receptor (ER)-negative with high metastatic potential, produce high levels of prostaglandin E2 (PGE2). In some cell types, expression of the inducible COX-2 isoform occurred in association with a ras gene mutation. We determined COX-1 and COX-2 expression, and the corresponding PGE2 secretions, in 4 ER-negative human breast cancer cell lines, the MCF10A breast epithelial cell line, and the same non-cancerous line transfected with a mutated ras gene. The highly invasive MDA-MB-231 and Hs578T cancer cell lines, which possess a mutated Ki-ras and H-ras, respectively, expressed constitutive and inducible COX-2, and produced high PGE2 levels; the less invasive MDA-MB-435 and SK-BR-3 lines, without a mutated ras, possessed only low levels of COX-2, and secreted correspondingly low PGE2 levels. Similarly, the ras transfectant, but not parental MCF10A cells, expressed inducible COX-2. Chemosuppression with a selective COX-2 inhibitor may be effective only in that minority of breast cancers which have a mutated ras gene.

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August 1999
Volume 15 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Gilhooly, E., & Gilhooly, E. (1999). The association between a mutated ras gene and cyclooxygenase-2 expression in human breast cancer cell lines.. International Journal of Oncology, 15, 267-337. https://doi.org/10.3892/ijo.15.2.267
MLA
Gilhooly, E., Rose, D."The association between a mutated ras gene and cyclooxygenase-2 expression in human breast cancer cell lines.". International Journal of Oncology 15.2 (1999): 267-337.
Chicago
Gilhooly, E., Rose, D."The association between a mutated ras gene and cyclooxygenase-2 expression in human breast cancer cell lines.". International Journal of Oncology 15, no. 2 (1999): 267-337. https://doi.org/10.3892/ijo.15.2.267