Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells.

  • Authors:
    • S Tatebe
    • T Matsuura
    • K Endo
    • K Teramachi
    • T Nakamura
    • K Sato
    • H Ito
  • View Affiliations

  • Published online on: August 1, 1999     https://doi.org/10.3892/ijo.15.2.229
  • Pages: 229-264
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Abstract

We examined the susceptibility of six human gastric carcinoma cell lines to infection with recombinant p53 adenovirus vector (AxCA-p53). AxCA-p53 infection at a muliplicity of infection (MOI) of 50 resulted in apoptotic cell death (MKN-1 cells), growth arrest (MKN-45, MKN-74 and KATO-III cells), or non-effectiveness (TMK-1 and OCUM-2M cells). Western blot analysis revealed increasing expression levels of p21/WAF1 protein after infection with AxCA-p53 in all the cell lines. After infection with AxCA-p53, the expression levels of bax or bcl-XL protein changed in MKN-1, but not in the other cell lines. These results suggest that the apoptotic pathway (dependence on the expressions of bcl-2 family proteins) dominates the growth arrest pathway (dependence on the expressions of p21/WAF1 protein) after infection with AxCA-p53. Thus, the bcl-2 family might play a crucial role in p53-mediated growth arrest and apoptosis in human gastric carcinoma cells.

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August 1999
Volume 15 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Tatebe, S., Matsuura, T., Endo, K., Teramachi, K., Nakamura, T., Sato, K., & Ito, H. (1999). Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells.. International Journal of Oncology, 15, 229-264. https://doi.org/10.3892/ijo.15.2.229
MLA
Tatebe, S., Matsuura, T., Endo, K., Teramachi, K., Nakamura, T., Sato, K., Ito, H."Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells.". International Journal of Oncology 15.2 (1999): 229-264.
Chicago
Tatebe, S., Matsuura, T., Endo, K., Teramachi, K., Nakamura, T., Sato, K., Ito, H."Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells.". International Journal of Oncology 15, no. 2 (1999): 229-264. https://doi.org/10.3892/ijo.15.2.229