Growth of HPV-18 immortalized human prostatic intraepithelial neoplasia cell lines. Influence of IL-10, follistatin, activin-A, and DHT.

  • Authors:
    • M Wang
    • A Liu
    • F U Garcia
    • J S Rhim
    • M E Stearns
  • View Affiliations

  • Published online on: June 1, 1999     https://doi.org/10.3892/ijo.14.6.1185
  • Pages: 1185-1280
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Abstract

Cultures from high grade prostatic intraepithelial neoplasia (HGPIN) have been established and immortalized by HPV-18 infection. The cultures were identified as PIN by Western blotting with anti-cytokeratin (34betaE12) and prostate specific antigen (PSA) antibodies. We examined the growth capabilities of the cultures in the presence of TGF-beta1, activin-A, follistatin (FS), androgens (DHEA, DHT) and several cytokines (IL-10, IL-2, IL-4). IL-10, FS, and DHT stimulated cell proliferation and colony forming ability, while the other cytokines and growth factors had no discernable effect. In addition, DHT and to a lesser extent IL-10 both stimulated PSA production. Activin-A blocked IL-10, FS, and DHT stimulated growth and PSA production. We interpret the data to mean that IL-10 induction of FS secretion (and FS binding of activin A) restores the normal growth capabilities of HGPIN cultures.

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Jun 1999
Volume 14 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Wang, M., Liu, A., Garcia, F., Rhim, J., & Stearns, M. (1999). Growth of HPV-18 immortalized human prostatic intraepithelial neoplasia cell lines. Influence of IL-10, follistatin, activin-A, and DHT.. International Journal of Oncology, 14, 1185-1280. https://doi.org/10.3892/ijo.14.6.1185
MLA
Wang, M., Liu, A., Garcia, F., Rhim, J., Stearns, M."Growth of HPV-18 immortalized human prostatic intraepithelial neoplasia cell lines. Influence of IL-10, follistatin, activin-A, and DHT.". International Journal of Oncology 14.6 (1999): 1185-1280.
Chicago
Wang, M., Liu, A., Garcia, F., Rhim, J., Stearns, M."Growth of HPV-18 immortalized human prostatic intraepithelial neoplasia cell lines. Influence of IL-10, follistatin, activin-A, and DHT.". International Journal of Oncology 14, no. 6 (1999): 1185-1280. https://doi.org/10.3892/ijo.14.6.1185