Insulin-like growth factor binding protein-3 does not mediate the interferon-dependent suppression of human ectocervical epithelial cell proliferation.

  • Authors:
    • J R Hembree
    • A Lambert
    • C Agarwal
    • T Efimova
    • R L Eckert
  • View Affiliations

  • Published online on: June 1, 1999     https://doi.org/10.3892/ijo.14.6.1163
  • Pages: 1163-1171
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Abstract

Interferon is a potential therapeutic agent for the treatment of cervical cancer. In the present study we examine the role of IFNgamma as a regulator of proliferation and production of IGFBP-3 expression in ectocervical epithelial cells. ECE16-1 cells are a model for studying early human papillomavirus-dependent cervical disease. IFNgamma produces a concentration-dependent inhibition of ECE16-1 cell proliferation that is associated with an increase in insulin-like growth factor binding protein-3 level. Growth suppression and IGFBP-3 increase is maximal at concentrations of IFNgamma >/=0.75 ng/ml. The increased IGFBP-3 expression is mediated via an increase in IGFBP-3 encoding mRNA. In contrast, IFNgamma inhibits proliferation of CaSki and SiHa cells, but IGFBP-3 is barely detectable and levels are not regulated by IFNgamma. These results suggest that the IFNgamma-dependent suppression of CaSki and SiHa cell proliferation is not mediated by secreted IGFBP-3. This result was confirmed when vector-mediated overexpression of immunoreactive IGFBP-3 in SiHa and CaSki cells did not consistently result in reduced cell proliferation rate.

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Jun 1999
Volume 14 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Hembree, J., Lambert, A., Agarwal, C., Efimova, T., & Eckert, R. (1999). Insulin-like growth factor binding protein-3 does not mediate the interferon-dependent suppression of human ectocervical epithelial cell proliferation.. International Journal of Oncology, 14, 1163-1171. https://doi.org/10.3892/ijo.14.6.1163
MLA
Hembree, J., Lambert, A., Agarwal, C., Efimova, T., Eckert, R."Insulin-like growth factor binding protein-3 does not mediate the interferon-dependent suppression of human ectocervical epithelial cell proliferation.". International Journal of Oncology 14.6 (1999): 1163-1171.
Chicago
Hembree, J., Lambert, A., Agarwal, C., Efimova, T., Eckert, R."Insulin-like growth factor binding protein-3 does not mediate the interferon-dependent suppression of human ectocervical epithelial cell proliferation.". International Journal of Oncology 14, no. 6 (1999): 1163-1171. https://doi.org/10.3892/ijo.14.6.1163