Gene therapy of chondrosarcoma using retrovirus vectors encoding the herpes simplex virus thymidine kinase gene.

  • Authors:
    • M Seto
    • H Wakabayashi
    • T Yamazaki
    • J Sonoda
    • Y Shinto
    • A Uchida
  • View Affiliations

  • Published online on: June 1, 1999     https://doi.org/10.3892/ijo.14.6.1137
  • Pages: 1137-1178
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Human chondrosarcoma cells (HCS-TG) were transduced with the gene for a herpes simplex virus thymidine kinase (HSV-tk) or Escherichia coli beta-galactosidase (lacZ). We investigated the cytotoxicity of human chondrosarcoma bearing an HSV-tk gene after treatment with ganciclovir. Chondrosarcoma cells bearing an HSV-tk gene were more sensitive than non-transduced cells. Coculturing with chondrosarcoma cells bearing both an HSV-tk gene (HCS-TG-tk) and lacZ gene (HCS-TG-Z) in various ratios showed a bystander effect. Chondrosarcoma implanted in nude mice were injected with HCS-TG-tk cells. After 4 weeks, the growth of tumors was significantly prevented.

Related Articles

Journal Cover

Jun 1999
Volume 14 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Seto, M., Wakabayashi, H., Yamazaki, T., Sonoda, J., Shinto, Y., & Uchida, A. (1999). Gene therapy of chondrosarcoma using retrovirus vectors encoding the herpes simplex virus thymidine kinase gene.. International Journal of Oncology, 14, 1137-1178. https://doi.org/10.3892/ijo.14.6.1137
MLA
Seto, M., Wakabayashi, H., Yamazaki, T., Sonoda, J., Shinto, Y., Uchida, A."Gene therapy of chondrosarcoma using retrovirus vectors encoding the herpes simplex virus thymidine kinase gene.". International Journal of Oncology 14.6 (1999): 1137-1178.
Chicago
Seto, M., Wakabayashi, H., Yamazaki, T., Sonoda, J., Shinto, Y., Uchida, A."Gene therapy of chondrosarcoma using retrovirus vectors encoding the herpes simplex virus thymidine kinase gene.". International Journal of Oncology 14, no. 6 (1999): 1137-1178. https://doi.org/10.3892/ijo.14.6.1137