Gene therapy of chondrosarcoma using retrovirus vectors encoding the herpes simplex virus thymidine kinase gene.
- M Seto
- H Wakabayashi
- T Yamazaki
- J Sonoda
- Y Shinto
- A Uchida
Affiliations: Department of Orthopaedic Surgery, Mie University School of Medicine, Tsu City, Mie Prefecture 514-8507, Japan.
- Published online on: June 1, 1999 https://doi.org/10.3892/ijo.14.6.1137
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Human chondrosarcoma cells (HCS-TG) were transduced with the gene for a herpes simplex virus thymidine kinase (HSV-tk) or Escherichia coli beta-galactosidase (lacZ). We investigated the cytotoxicity of human chondrosarcoma bearing an HSV-tk gene after treatment with ganciclovir. Chondrosarcoma cells bearing an HSV-tk gene were more sensitive than non-transduced cells. Coculturing with chondrosarcoma cells bearing both an HSV-tk gene (HCS-TG-tk) and lacZ gene (HCS-TG-Z) in various ratios showed a bystander effect. Chondrosarcoma implanted in nude mice were injected with HCS-TG-tk cells. After 4 weeks, the growth of tumors was significantly prevented.