Retroviruses prepared from human DAF expressing murine packaging cells acquire resistance against human serum.

  • Authors:
    • A Hiasa
    • M Watanabe
    • H Okada
    • K Ikenaka
    • T Fujita
    • T Yoshimatsu
    • T Kanematsu
    • H Shiku
  • View Affiliations

  • Published online on: June 1, 1999     https://doi.org/10.3892/ijo.14.6.1091
  • Pages: 1091-1097
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Abstract

The complement system of the human body inactivates the infectious ability of retroviruses injected as an artificial gene transfer vector. We established new murine leukemia virus (MuLV) packaging cell lines; D2SS and D7S which express decay-accelerating factor (DAF) on their surface. Both D2SS and D7S were resistant against incubation with fresh human serum. Moreover, the retroviruses produced from these packaging cell lines were also resistant to serum treatment. This resistance can be inhibited by DAF neutralizing antibody 1C6. These data demostrate that DAF induces a partial protection of MuLV infection from the human complement system.

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Jun 1999
Volume 14 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Hiasa, A., Watanabe, M., Okada, H., Ikenaka, K., Fujita, T., Yoshimatsu, T. ... Shiku, H. (1999). Retroviruses prepared from human DAF expressing murine packaging cells acquire resistance against human serum.. International Journal of Oncology, 14, 1091-1097. https://doi.org/10.3892/ijo.14.6.1091
MLA
Hiasa, A., Watanabe, M., Okada, H., Ikenaka, K., Fujita, T., Yoshimatsu, T., Kanematsu, T., Shiku, H."Retroviruses prepared from human DAF expressing murine packaging cells acquire resistance against human serum.". International Journal of Oncology 14.6 (1999): 1091-1097.
Chicago
Hiasa, A., Watanabe, M., Okada, H., Ikenaka, K., Fujita, T., Yoshimatsu, T., Kanematsu, T., Shiku, H."Retroviruses prepared from human DAF expressing murine packaging cells acquire resistance against human serum.". International Journal of Oncology 14, no. 6 (1999): 1091-1097. https://doi.org/10.3892/ijo.14.6.1091