Proliferating cell nuclear antigen as a predictor of therapeutic effect of continuous 5-fluorouracil administration in gastric cancer.

  • Authors:
    • Y Iwatani
    • T Kamigaki
    • S Suzuki
    • M Ohno
    • T Nakamura
    • Y Kuroda
  • View Affiliations

  • Published online on: May 1, 1999     https://doi.org/10.3892/ijo.14.5.965
  • Pages: 965-1035
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Abstract

The change of proliferating cell nuclear antigen (PCNA) expression was examined in three gastric cancer cell lines, MKN28, MKN45 and MKN74, during continuous or bolus exposure to 5-fluorouracil (5-FU). The cytotoxic effect of 5-FU was almost the same in all the cells. PCNA expression was higher at 24 and 72 h after continuous 5-FU treatment than before treatment. Continuous 5-FU treatment of cells revealed higher expression of PCNA protein and mRNA than did bolus treatment. Flow cytometry revealed that 5-FU increased cell population at the late G1 or S phase 24 and 72 h after treatment. PCNA values at the late G1 and S phase were significantly higher than those at other phases 24 h after treatment. These results suggest that PCNA expression increased due to cell cycle accumulation at late G1 and S phases. Thus PCNA values just after chemotherapy of gastric cancer may be useful in predicting the therapeutic effects of continuous 5-FU administration.

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May 1999
Volume 14 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Iwatani, Y., Kamigaki, T., Suzuki, S., Ohno, M., Nakamura, T., & Kuroda, Y. (1999). Proliferating cell nuclear antigen as a predictor of therapeutic effect of continuous 5-fluorouracil administration in gastric cancer.. International Journal of Oncology, 14, 965-1035. https://doi.org/10.3892/ijo.14.5.965
MLA
Iwatani, Y., Kamigaki, T., Suzuki, S., Ohno, M., Nakamura, T., Kuroda, Y."Proliferating cell nuclear antigen as a predictor of therapeutic effect of continuous 5-fluorouracil administration in gastric cancer.". International Journal of Oncology 14.5 (1999): 965-1035.
Chicago
Iwatani, Y., Kamigaki, T., Suzuki, S., Ohno, M., Nakamura, T., Kuroda, Y."Proliferating cell nuclear antigen as a predictor of therapeutic effect of continuous 5-fluorouracil administration in gastric cancer.". International Journal of Oncology 14, no. 5 (1999): 965-1035. https://doi.org/10.3892/ijo.14.5.965