Anti-proliferating effects of ginsenoside Rh2 on MCF-7 human breast cancer cells.

  • Authors:
    • M Oh
    • Y H Choi
    • S Choi
    • H Chung
    • K Kim
    • S I Kim
    • D K Kim
    • N D Kim
  • View Affiliations

  • Published online on: May 1, 1999     https://doi.org/10.3892/ijo.14.5.869
  • Pages: 869-944
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Abstract

Ginsenoside Rh2 (G-Rh2) isolated from the root of Panax ginseng has been shown to have anti-cancer proliferation, differentiation and chemopreventive effects in certain cancer cell types. We investigated the mechanism of G-Rh2-induced growth inhibition in MCF-7 human breast carcinoma cells. G-Rh2 significantly inhibited the cell growth in a concentration-dependent manner, which effect was reversible, and induced a G1 arrest in cell cycle progression. G-Rh2 treatment down-regulated the protein level of cyclin D3 but upregulated the expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1. The increased levels of p21 were associated with increased binding of p21 and Cdk2 concomitant with marked decrease in Cdk2 and cyclin E-dependent kinase activities with no changes in Cdk2 and cyclin E expression. G-Rh2 markedly reduced the phosphorylated retinoblastoma protein (pRb) and enchanced association of unphosphorylated pRb and the transcription factor E2F-1. These data suggest that G-Rh2 inhibited the growth of MCF-7 cells, by inducing protein expression of p21 and reducing the protein levels of cyclin D which resulted in the down-regulation of cyclin/Cdk complex kinase activity, decreasing phosphorylation of pRb, and inhibiting E2F release.

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May 1999
Volume 14 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Oh, M., Choi, Y., Choi, S., Chung, H., Kim, K., Kim, S. ... Kim, N. (1999). Anti-proliferating effects of ginsenoside Rh2 on MCF-7 human breast cancer cells.. International Journal of Oncology, 14, 869-944. https://doi.org/10.3892/ijo.14.5.869
MLA
Oh, M., Choi, Y., Choi, S., Chung, H., Kim, K., Kim, S., Kim, D., Kim, N."Anti-proliferating effects of ginsenoside Rh2 on MCF-7 human breast cancer cells.". International Journal of Oncology 14.5 (1999): 869-944.
Chicago
Oh, M., Choi, Y., Choi, S., Chung, H., Kim, K., Kim, S., Kim, D., Kim, N."Anti-proliferating effects of ginsenoside Rh2 on MCF-7 human breast cancer cells.". International Journal of Oncology 14, no. 5 (1999): 869-944. https://doi.org/10.3892/ijo.14.5.869