Interphase cytogenetic diagnosis of bladder cancer on cells from urine and bladder washing.

  • Authors:
    • K Junker
    • W Werner
    • C Mueller
    • W Ebert
    • J Schubert
    • U Claussen
  • View Affiliations

  • Published online on: February 1, 1999     https://doi.org/10.3892/ijo.14.2.309
  • Pages: 309-322
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In order to determine the value of fluorescence in situ hybridization (FISH) in the diagnosis and follow-up of bladder cancer interphase cytogenetics was performed on cells from urine and bladder washings. 50 ml of urine or bladder washings were collected. FISH was carried out using centromere probes for chromosomes 7, 8, 9 and 12 according to standard protocols. In each case 100 cell nuclei were analysed. Fifty-four samples from urine and 67 samples from bladder washing were analysed by FISH in comparison with results obtained by conventional cytology. Sensitivity of detection of tumor cells by FISH was 68.5% in urine and 63% in bladder washings regardless of tumor stage and grade. Sensitivity obtained by conventional cytology was 50% in urine and 77.3% in bladder washings. FISH on cells from urine samples is an effective complement to the standard urine cytology. Using centromere probes this approach is characterized by high specificity and sensitivity in tumors with T-category higher than pTa and grade higher than G1.

Related Articles

Journal Cover

February 1999
Volume 14 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Junker, K., Werner, W., Mueller, C., Ebert, W., Schubert, J., & Claussen, U. (1999). Interphase cytogenetic diagnosis of bladder cancer on cells from urine and bladder washing.. International Journal of Oncology, 14, 309-322. https://doi.org/10.3892/ijo.14.2.309
MLA
Junker, K., Werner, W., Mueller, C., Ebert, W., Schubert, J., Claussen, U."Interphase cytogenetic diagnosis of bladder cancer on cells from urine and bladder washing.". International Journal of Oncology 14.2 (1999): 309-322.
Chicago
Junker, K., Werner, W., Mueller, C., Ebert, W., Schubert, J., Claussen, U."Interphase cytogenetic diagnosis of bladder cancer on cells from urine and bladder washing.". International Journal of Oncology 14, no. 2 (1999): 309-322. https://doi.org/10.3892/ijo.14.2.309