Killing effects of 5-fluorouracil on human biliary tract cancer cell lines.

  • Authors:
    • Y Moon
    • T Todoroki
    • T Ohno
    • K Fukao
    • J B Little
  • View Affiliations

  • Published online on: February 1, 1999     https://doi.org/10.3892/ijo.14.2.253
  • Pages: 253-260
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Data concerning the cellular sensitivity of human biliary tract cancer cell lines to 5-FU are scarce. The purpose of the present study was to evaluate the cellular sensitivity of Mz-ChA-2 (derived from gall bladder cancer) and SK-ChA-1 (derived from bile duct cancer) to 5-FU. The clonogenic capacity of these lines after pulse (1 h) or continuous (168 h) administration of various 5-FU concentrations (0.1-100 microg/ml) was evaluated in exponentially growing cells as well as in those that had reached the plateau phase. In both cell lines, exponentially growing cells were 1.8-times more susceptible to 5-FU than those in plateau phase. However, when cells in the same growth phase were compared, Mz-ChA-2 cells were 10 times more sensitive to the drug than SK-ChA-1 cells. Regardless of the growth phase or cell line, increasing the duration of exposure to 5-FU decreased the proportion of surviving cells. Even at a non-cytocidal dosage, exposing the cells for three doubling times markedly decreased the number of viable cells remaining after treatment.

Related Articles

Journal Cover

February 1999
Volume 14 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Moon, Y., Todoroki, T., Ohno, T., Fukao, K., & Little, J. (1999). Killing effects of 5-fluorouracil on human biliary tract cancer cell lines.. International Journal of Oncology, 14, 253-260. https://doi.org/10.3892/ijo.14.2.253
MLA
Moon, Y., Todoroki, T., Ohno, T., Fukao, K., Little, J."Killing effects of 5-fluorouracil on human biliary tract cancer cell lines.". International Journal of Oncology 14.2 (1999): 253-260.
Chicago
Moon, Y., Todoroki, T., Ohno, T., Fukao, K., Little, J."Killing effects of 5-fluorouracil on human biliary tract cancer cell lines.". International Journal of Oncology 14, no. 2 (1999): 253-260. https://doi.org/10.3892/ijo.14.2.253