Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.

  • Authors:
    • J Ellerhorst
    • T Nguyen
    • D N Cooper
    • Y Estrov
    • D Lotan
    • R Lotan
  • View Affiliations

  • Published online on: February 1, 1999     https://doi.org/10.3892/ijo.14.2.225
  • Pages: 225-257
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Galectin-1 has been implicated in the process of vertebrate developmental regulation. Sodium butyrate is an established differentiation-inducing agent and has been shown to increase galectin-1 expression in colon carcinoma cells. We studied the roles of butyrate and galectin-1 in the induction of differentiation and apoptosis in the prostate cancer cell line LNCaP. Treatment of LNCaP cells with butyrate resulted in induction of galectin-1 expression in a time- and dose-dependent manner. Treatment with butyrate also resulted in inhibition of proliferation, morphologic changes consistent with a differentiated phenotype, and induction of apoptosis. Prostate specific antigen expression was transiently reduced. To determine which of these effects might be secondary to the induction of galectin-1, LNCaP cells were transfected with a galectin-1 expression vector. The transfected cells displayed growth inhibition and an increased rate of apoptosis. PSA expression was not affected. We conclude that galectin-1 may be responsible for many of the phenotypic changes resulting from butyrate treatment and may function downstream in the pathway of butyrate-induced differentiation. We also found PSA to be somewhat inconsistent as an indicator of differentiation of LNCaP cells, likely due to other factors influencing its expression.

Related Articles

Journal Cover

February 1999
Volume 14 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Ellerhorst, J., Nguyen, T., Cooper, D., Estrov, Y., Lotan, D., & Lotan, R. (1999). Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.. International Journal of Oncology, 14, 225-257. https://doi.org/10.3892/ijo.14.2.225
MLA
Ellerhorst, J., Nguyen, T., Cooper, D., Estrov, Y., Lotan, D., Lotan, R."Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.". International Journal of Oncology 14.2 (1999): 225-257.
Chicago
Ellerhorst, J., Nguyen, T., Cooper, D., Estrov, Y., Lotan, D., Lotan, R."Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.". International Journal of Oncology 14, no. 2 (1999): 225-257. https://doi.org/10.3892/ijo.14.2.225