Differential expression of endogenous galectin-1 and galectin-3 in human prostate cancer cell lines and effects of overexpressing galectin-1 on cell phenotype.

  • Authors:
    • J Ellerhorst
    • T Nguyen
    • D N Cooper
    • D Lotan
    • R Lotan
  • View Affiliations

  • Published online on: February 1, 1999     https://doi.org/10.3892/ijo.14.2.217
  • Pages: 217-241
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Abstract

We have analyzed the expression of galectin-1 and galectin-3 in four human prostate carcinoma cell lines. Northern analysis and immunoblotting experiments showed that three cell lines express both galectins. However, only galectin-1 was detected on the surface of these cells. The LNCaP line expressed neither galectin. LNCaP was transfected with galectin-1 and four clones were isolated, all of which expressed galectin-1 on the cell surface. Kinetics of binding to extracellular matrix proteins appeared to be accelerated in the transfected lines, but overall binding was not enhanced. When the same experiments were performed in the presence of EDTA to eliminate the effects of integrins, binding of a galectin-1 clone to laminin and fibronectin was increased relative to the control cell line. We propose that galectins may contribute to the adhesive properties of some prostate cancer cells.

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February 1999
Volume 14 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ellerhorst, J., Nguyen, T., Cooper, D., Lotan, D., & Lotan, R. (1999). Differential expression of endogenous galectin-1 and galectin-3 in human prostate cancer cell lines and effects of overexpressing galectin-1 on cell phenotype.. International Journal of Oncology, 14, 217-241. https://doi.org/10.3892/ijo.14.2.217
MLA
Ellerhorst, J., Nguyen, T., Cooper, D., Lotan, D., Lotan, R."Differential expression of endogenous galectin-1 and galectin-3 in human prostate cancer cell lines and effects of overexpressing galectin-1 on cell phenotype.". International Journal of Oncology 14.2 (1999): 217-241.
Chicago
Ellerhorst, J., Nguyen, T., Cooper, D., Lotan, D., Lotan, R."Differential expression of endogenous galectin-1 and galectin-3 in human prostate cancer cell lines and effects of overexpressing galectin-1 on cell phenotype.". International Journal of Oncology 14, no. 2 (1999): 217-241. https://doi.org/10.3892/ijo.14.2.217