Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.

  • Authors:
    • T Aikoh
    • A Tomokuni
    • T Matsukii
    • F Hyodoh
    • H Ueki
    • T Otsuki
    • A Ueki
  • View Affiliations

  • Published online on: June 1, 1998     https://doi.org/10.3892/ijo.12.6.1355
  • Pages: 1355-1364
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Silica and related substances such as silicate have been proven to possess "adjuvant effects". We have previously reported a finding of polyclonal human T cell activation induced by silicate as a superantigen in vitro. In this study, we observed activation-induced cell death in human lymphocytes after stimulation with chrysotile, a kind of silicate. Apoptotic cells were detected flow cytometrically using the TUNEL assay, and the maximum appearance of TUNEL positive cells occurred on day 4 of incubation. Simultaneously the manifestation of small-sized cells in the specimens increased implying apoptosis. Fas expression on lymphocytes increased to day 3 of incubation with chrysotile, and then spontaneously decreased on day 4 when remarkable apoptosis could be detected. Based on these results it is conceivable that activation-induced cell death occurred through Fas-Fas ligand interaction in lymphocytes after stimulation with silicate in a concentration with which no acute cytotoxicity has been detected. Whether and how the repeated apoptosis in definite clones of lymphocytes causes the induction of sFas synthesis need clarification.

Related Articles

Journal Cover

June 1998
Volume 12 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Aikoh, T., Tomokuni, A., Matsukii, T., Hyodoh, F., Ueki, H., Otsuki, T., & Ueki, A. (1998). Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.. International Journal of Oncology, 12, 1355-1364. https://doi.org/10.3892/ijo.12.6.1355
MLA
Aikoh, T., Tomokuni, A., Matsukii, T., Hyodoh, F., Ueki, H., Otsuki, T., Ueki, A."Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.". International Journal of Oncology 12.6 (1998): 1355-1364.
Chicago
Aikoh, T., Tomokuni, A., Matsukii, T., Hyodoh, F., Ueki, H., Otsuki, T., Ueki, A."Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.". International Journal of Oncology 12, no. 6 (1998): 1355-1364. https://doi.org/10.3892/ijo.12.6.1355