Clinical significance of pyrimidine nucleoside phosphorylase in colorectal carcinoma
- K Mimori
- M Mori
- T Shiraishi
- M Haraguchi
- H Ueo
- T Akiyoshi
Affiliations: KYUSHU UNIV,MED INST BIOREGULAT,DEPT SURG,BEPPU,OITA 874,JAPAN. OITA PREFECTURAL HOSP,DEPT SURG,OITA,JAPAN.
- Published online on: March 1, 1997 https://doi.org/10.3892/ijo.10.3.493
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The expression of pyrimidine nucleoside phosphorylase (PyNPase) mRNA in tumor (T) and normal (N) biopsy specimens obtained from 41 cases of colorectal carcinoma was examined by the reverse transcriptase/polymerase chain reaction (RT-PCR). The higher T/N ratio of the expression of PyNPase mRNA correlated significantly with the presence of lymph vessel invasion (p=0.039), the positive lymph node metastasis (p=0.014) and the advanced stage of the disease (p=0.014). There was a significant con-elation between the results determined by enzyme activity and those determined by RT-PCR (p=0.005). The findings suggested that the determination of PyNPase mRNA by RT-PCR may give useful information on tumor aggressiveness of colorectal carcinoma and this method can be used instead of enzyme activity.