Frequent expression of MDR1 and MDR3 genes in acute myelocytic leukemia cells with t(8;21) (q22;q22)

  • Authors:
    • M Mizutani
    • M Yamaguchi
    • H Miwa
    • T Kawamura
    • T Okuno
    • K Nishii
    • H Shiku
    • N Kamada
    • K Kita
  • View Affiliations

  • Published online on: March 1, 1997     https://doi.org/10.3892/ijo.10.3.473
  • Pages: 473-479
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Abstract

Multidrug resistance (MDR) 1 and MDR3 gene expression were examined in acute myelocytic leukemia (AML) cells from 126 Japanese patients. In 119 AML patients at diagnosis 52 were revealed to have P-gp/MDR1 (43.7%). AML cases with t(8;21) had a higher incidence of P-gp expression (13/17; 76.5%) than cases with other karyotypes (33/89; 37.1%) (p=0.0062). CD19(+) cases expressed P-gp frequently (17/25) as did CD7(+) cases (24/35). In 17 CD19(+) AML with P-gp expression, 12 had t(8;21) abnormality. In 68 AML samples examined, MDR3 mRNA was detected in 11 cases, 9 of which had the t(8;21) abnormality. The MDR3(+) t(8;21) AML samples were also positive for CD19. We analyzed P-gp expression at both diagnosis and relapse in 18 AML patients. All 11 P-gp(+) cases at relapse, in which 5 patients were P-gp negative at diagnosis, showed either t(8;21) or CD7 positivity. Our data demonstrated that expression of MDR1 and MDR3 in AML is closely associated with chromosome abnormality t(8;21) and expression of immature lymphoid antigen CD19 as well as CD7. Kasumi-1, a t(8;21) AML cell line, was demonstrated to lose P-gp by the treatment of 1,25(OH)(2)D-3, a monocyte/macropharge differentiation inducer, suggesting the possible contribution to the therapy for AML.

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March 1997
Volume 10 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Mizutani, M., Yamaguchi, M., Miwa, H., Kawamura, T., Okuno, T., Nishii, K. ... Kita, K. (1997). Frequent expression of MDR1 and MDR3 genes in acute myelocytic leukemia cells with t(8;21) (q22;q22). International Journal of Oncology, 10, 473-479. https://doi.org/10.3892/ijo.10.3.473
MLA
Mizutani, M., Yamaguchi, M., Miwa, H., Kawamura, T., Okuno, T., Nishii, K., Shiku, H., Kamada, N., Kita, K."Frequent expression of MDR1 and MDR3 genes in acute myelocytic leukemia cells with t(8;21) (q22;q22)". International Journal of Oncology 10.3 (1997): 473-479.
Chicago
Mizutani, M., Yamaguchi, M., Miwa, H., Kawamura, T., Okuno, T., Nishii, K., Shiku, H., Kamada, N., Kita, K."Frequent expression of MDR1 and MDR3 genes in acute myelocytic leukemia cells with t(8;21) (q22;q22)". International Journal of Oncology 10, no. 3 (1997): 473-479. https://doi.org/10.3892/ijo.10.3.473