Possible involvement of protein phosphatase type 2A in tumor necrosis factor alpha-induced tissue factor expression in endothelial cells

  • Authors:
    • S Nagaya
    • K Nakai
    • M Shimura
    • Y Suzuki
    • H Shiku
    • M Nishikawa
  • View Affiliations

  • Published online on: March 1, 1997     https://doi.org/10.3892/ijo.10.3.443
  • Pages: 443-448
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Abstract

The involvement of protein Ser/Thr phosphatase types 2A (PP2A) and 1 (PP1) in tumor necrosis factor alpha (TNF alpha)-induced tissue factor (TF) expression of human umbilical vein endothelial cells (HUVEC) was investigated. PP2A was more abundant than PP1 in the cytosol of HUVEC. CAL-A (0.5 nM) and OKA (2 nM), cell permeable inhibitors of PP1 and PP2A, augmented TNF alpha-induced TF expression, although TF expression induced by either TPA or thrombin was unchanged in the presence of GAL-A. Addition of CAL-A (0.5 nM) to TNF alpha-stimulated cultures led to an increase in the accumulation of TF transcripts. CAL-A (0.5 nM) also augmented the TNF alpha-induced phosphorylation of I kappa B-alpha, an inhibitor of NF-kappa B. Since PP2A is more sensitive to OKA than PP1, these results suggest that PP2A may be involved in regulating TNF alpha-induced TF expression in HUVEC through I kappa B-alpha dephosphorylation.

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March 1997
Volume 10 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Nagaya, S., Nakai, K., Shimura, M., Suzuki, Y., Shiku, H., & Nishikawa, M. (1997). Possible involvement of protein phosphatase type 2A in tumor necrosis factor alpha-induced tissue factor expression in endothelial cells. International Journal of Oncology, 10, 443-448. https://doi.org/10.3892/ijo.10.3.443
MLA
Nagaya, S., Nakai, K., Shimura, M., Suzuki, Y., Shiku, H., Nishikawa, M."Possible involvement of protein phosphatase type 2A in tumor necrosis factor alpha-induced tissue factor expression in endothelial cells". International Journal of Oncology 10.3 (1997): 443-448.
Chicago
Nagaya, S., Nakai, K., Shimura, M., Suzuki, Y., Shiku, H., Nishikawa, M."Possible involvement of protein phosphatase type 2A in tumor necrosis factor alpha-induced tissue factor expression in endothelial cells". International Journal of Oncology 10, no. 3 (1997): 443-448. https://doi.org/10.3892/ijo.10.3.443