Interaction of p53 with protein kinase CK2 during SV40 induced entrance of quiescent cells into the cell cycle

  • Authors:
    • M Koenig
    • D Sturm
    • A Prowald
    • O Issinger
    • M Montenarh
  • View Affiliations

  • Published online on: February 1, 1997     https://doi.org/10.3892/ijo.10.2.405
  • Pages: 405-411
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Abstract

Quiescent non-permissive cells re-enter the cell cycle upon infection with the DNA tumor virus SV40. Before the expression of virus specific proteins and other cellular reactions there is an induced expression of the growth suppressor protein p53. p53 is known to be a substrate for protein kinase CK2 and in addition it is tightly associated with CK2 and both proteins are implicated in cell cycle regulation. No phosphorylation of p53 was observed in vivo until late in G(1)- or early S-phase. Immunopurified p53 from the early G(1)-phase of the cell cycle was not phosphorylated by an associated protein kinase activity. Furthermore, protein kinase CK2 could not phosphorylate p53 from the early G(1)-phase of the cell cycle and also immunopurified p53 from late G(1)- and S-phase which were dephosphorylated by alkaline and acid phosphatases. p53 from cells in S-phase is an efficient substrate. Moreover, in the presence of okadaic acid, a potent inhibitor of protein phosphatase PP2a, phosphorylation of p53 is detectable early in G(1)-phase of the cell cycle.

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February 1997
Volume 10 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Koenig, M., Sturm, D., Prowald, A., Issinger, O., & Montenarh, M. (1997). Interaction of p53 with protein kinase CK2 during SV40 induced entrance of quiescent cells into the cell cycle. International Journal of Oncology, 10, 405-411. https://doi.org/10.3892/ijo.10.2.405
MLA
Koenig, M., Sturm, D., Prowald, A., Issinger, O., Montenarh, M."Interaction of p53 with protein kinase CK2 during SV40 induced entrance of quiescent cells into the cell cycle". International Journal of Oncology 10.2 (1997): 405-411.
Chicago
Koenig, M., Sturm, D., Prowald, A., Issinger, O., Montenarh, M."Interaction of p53 with protein kinase CK2 during SV40 induced entrance of quiescent cells into the cell cycle". International Journal of Oncology 10, no. 2 (1997): 405-411. https://doi.org/10.3892/ijo.10.2.405