A human xenograft model for testing early events of epithelial neoplastic invasion

  • Authors:
    • J McCandless
    • A Cress
    • I Rabinovitz
    • C Payne
    • G Bowden
    • J Knox
    • R Nagle
  • View Affiliations

  • Published online on: February 1, 1997     https://doi.org/10.3892/ijo.10.2.279
  • Pages: 279-285
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Abstract

We report on a model of human prostate tumor cell invasion using the SCID (severe combined immunodeficient) mouse diaphragm. Tumor cells were injected into SCID mice intraperitoneally and the diaphragms harvested three to five weeks later. Electron microscopy showed tumor cell penetration of the mesothelial cell layer and adhesion to the underlying basement membrane on the inferior surface of the mouse diaphragm, where colonies developed. Immunohistochemistry showed invasion by tumor cells through the basement membrane into the muscle of the diaphragm, presence of human tumor cells among the muscle cells and the presence of selected proteins on the invasion front of the tumor cells. Digital image analysis enabled quantitative comparison of events in the metastatic cascade by variants of the tumor cell line and evaluation of the effectiveness of a putative tumor inhibitor. Results suggest that the SCID mouse diaphragm model is a convenient, effective, easily oriented and reproducible in vivo model of the early events associated with human prostate tumor cell invasion.

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February 1997
Volume 10 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
McCandless, J., Cress, A., Rabinovitz, I., Payne, C., Bowden, G., Knox, J., & Nagle, R. (1997). A human xenograft model for testing early events of epithelial neoplastic invasion. International Journal of Oncology, 10, 279-285. https://doi.org/10.3892/ijo.10.2.279
MLA
McCandless, J., Cress, A., Rabinovitz, I., Payne, C., Bowden, G., Knox, J., Nagle, R."A human xenograft model for testing early events of epithelial neoplastic invasion". International Journal of Oncology 10.2 (1997): 279-285.
Chicago
McCandless, J., Cress, A., Rabinovitz, I., Payne, C., Bowden, G., Knox, J., Nagle, R."A human xenograft model for testing early events of epithelial neoplastic invasion". International Journal of Oncology 10, no. 2 (1997): 279-285. https://doi.org/10.3892/ijo.10.2.279