Phenotypes of human esophageal squamous cell carcinoma based on matrix metalloproteinase production
- N Arima
- I Shima
- S Shimajiri
- Y Sasaguri
- T Sasaguri
- A Tanimoto
- T Hamada
- M Morimatsu
Affiliations: UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT PATHOL,YAHATANISHI KU,KITAKYUSHU,FUKUOKA 807,JAPAN. KURUME UNIV,SCH MED,DEPT SURG,KURUME,FUKUOKA 830,JAPAN. KURUME UNIV,SCH MED,DEPT PATHOL,KURUME,FUKUOKA 830,JAPAN.
- Published online on: February 1, 1997 https://doi.org/10.3892/ijo.10.2.269
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We investigated the effects of epidermal growth factor (EGF) and transforming growth factor-beta (TGF-beta) on cell growth and on regulation of matrix metalloproteinases (MMPs) in four cell lines of human esophageal squamous cell carcinomas (TE8, TE9, TE10, and TE11). EGF stimulated the production of proforms of gelatinase B (MMP-9) by three cell lines that could synthesize EGF by themselves, with TE9 being the exception. Particularly, both the production of MMP-9 and DNA synthesis in TE10 were stimulated significantly by EGF. TGF-beta slightly stimulated DNA synthesis in two cell lines, TE9 and TE11, and TGF-beta secretion by TE9 was detected. The production of proforms of gelatinases A (MMP-2) and MMP-9 was gradually induced by TGF-beta in a concentration-dependent manner in all the cell lines except for TE9. Flow cytometric analysis revealed that all the lines expressed both EGF- and TGF-beta-receptors. In conclusion, our present results indicate that at least there are possibly two distinct phenotypes in human esophageal squamous cell carcinoma: one (TE10) depends on autocrine EGF production that enhances DNA synthesis and MMP-9 production; and the other (TE9) on autocrine TGF-beta that stimulates DNA synthesis but not in relation to gelatinase production.