INTRACELLULAR FREE FATTY-ACID RELEASE AND LIPID-PEROXIDATION IN CULTURED HUMAN BREAST-CANCER CELLS IN RESPONSE TO GAMMA-LINOLENIC ACID WITH IRON (GLA + FE)

  • Authors:
    • S TAKEDA
    • PG SIM
    • DF HORROBIN
    • KA CHISHOLM
    • VA SIMMONS
    • GW ELLS
    • DK JENKINS
    • NL MORSEFISHER
  • View Affiliations

  • Published online on: December 1, 1992     https://doi.org/10.3892/ijo.1.7.759
  • Pages: 759-763
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Abstract

Intracellular free fatty acid (FFA) release and peroxidation of polyunsaturated fatty acid (PUFA) were studied in cultured human breast cancer cells (ZR-75-1) exposed to gamma-linolenic acid with iron (GLA + Fe). This treatment results in cell death. Increased intracellular FFA were observed in association with both the accelerated peroxidation of PUFA and the killing effect. Vitamin E reduced all three effects. The FFA were methyl esterified and analyzed by gas chromatography-mass spectrometry. The identified FFAs were 16: 0, 18: 3, 20: 3 and 20: 4 (numbers of carbons and double bonds indicated). These results suggest an association of intracellular FFA release with the peroxidation of PUFA and the cancer cell-killing by GLA in the presence of iron.

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December 1992
Volume 1 Issue 7

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
TAKEDA, S., SIM, P., HORROBIN, D., CHISHOLM, K., SIMMONS, V., ELLS, G. ... MORSEFISHER, N. (1992). INTRACELLULAR FREE FATTY-ACID RELEASE AND LIPID-PEROXIDATION IN CULTURED HUMAN BREAST-CANCER CELLS IN RESPONSE TO GAMMA-LINOLENIC ACID WITH IRON (GLA + FE). International Journal of Oncology, 1, 759-763. https://doi.org/10.3892/ijo.1.7.759
MLA
TAKEDA, S., SIM, P., HORROBIN, D., CHISHOLM, K., SIMMONS, V., ELLS, G., JENKINS, D., MORSEFISHER, N."INTRACELLULAR FREE FATTY-ACID RELEASE AND LIPID-PEROXIDATION IN CULTURED HUMAN BREAST-CANCER CELLS IN RESPONSE TO GAMMA-LINOLENIC ACID WITH IRON (GLA + FE)". International Journal of Oncology 1.7 (1992): 759-763.
Chicago
TAKEDA, S., SIM, P., HORROBIN, D., CHISHOLM, K., SIMMONS, V., ELLS, G., JENKINS, D., MORSEFISHER, N."INTRACELLULAR FREE FATTY-ACID RELEASE AND LIPID-PEROXIDATION IN CULTURED HUMAN BREAST-CANCER CELLS IN RESPONSE TO GAMMA-LINOLENIC ACID WITH IRON (GLA + FE)". International Journal of Oncology 1, no. 7 (1992): 759-763. https://doi.org/10.3892/ijo.1.7.759