INTRACELLULAR FREE FATTY-ACID RELEASE AND LIPID-PEROXIDATION IN CULTURED HUMAN BREAST-CANCER CELLS IN RESPONSE TO GAMMA-LINOLENIC ACID WITH IRON (GLA + FE)
- S TAKEDA
- PG SIM
- DF HORROBIN
- KA CHISHOLM
- VA SIMMONS
- GW ELLS
- DK JENKINS
- NL MORSEFISHER
Affiliations: NATL RES COUNCIL CANADA,ATLANTIC REG LAB,INST MARINE BIOSCI,HALIFAX B3H 3Z1,NS,CANADA.
- Published online on: December 1, 1992 https://doi.org/10.3892/ijo.1.7.759
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Intracellular free fatty acid (FFA) release and peroxidation of polyunsaturated fatty acid (PUFA) were studied in cultured human breast cancer cells (ZR-75-1) exposed to gamma-linolenic acid with iron (GLA + Fe). This treatment results in cell death. Increased intracellular FFA were observed in association with both the accelerated peroxidation of PUFA and the killing effect. Vitamin E reduced all three effects. The FFA were methyl esterified and analyzed by gas chromatography-mass spectrometry. The identified FFAs were 16: 0, 18: 3, 20: 3 and 20: 4 (numbers of carbons and double bonds indicated). These results suggest an association of intracellular FFA release with the peroxidation of PUFA and the cancer cell-killing by GLA in the presence of iron.