Open Access

Dormancy and NKG2D system in brain metastases: Analysis of immunogenicity

  • Authors:
    • Charlotte Flüh
    • Victor Mafael
    • Vivian Adamski
    • Michael Synowitz
    • Janka Held‑Feindt
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  • Published online on: December 30, 2019     https://doi.org/10.3892/ijmm.2019.4449
  • Pages: 298-314
  • Copyright: © Flüh et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Patients with breast cancer (BC) and lung cancer (LC) are prone to developing brain metastases, which are associated with devastating prognoses. Dormant tumor cells, a population of non‑apoptotic quiescent cells and immunological escape mechanisms, including the Natural Killer Group 2 member D (NKG2D) receptor‑ligand system, represent potential mechanisms of tumor recurrence. To date, the immunological characteristics of dormant tumor cells concerning the NKG2D system in cerebral malignancies are mostly unknown. In the present study, an extensive characterization of dormant and NKG2D ligand (NKG2DL)+ cells in cerebral metastases was performed. The expression profiles and localization patterns of various NKG2DL and several dormancy markers were analyzed in solid human brain metastases from patients with BC and LC using immunostaining and reverse transcription‑quantitative polymerase chain reaction analyses. Statistical analysis was performed using Student's t‑test and Bravais‑Pearson correlation analysis. Not only ‘peripheral’, but also ‘central’ dormancy markers, which had been previously described in primary brain tumors, were identified in all cerebral metastases at detectable levels at protein and mRNA levels. Notably, the majority of NKG2DL+ cells were also positive for ‘central’ dormancy markers, but not ‘peripheral’ dormancy markers in both patient groups. This cell population may represent a promising future therapeutic target.
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February 2020
Volume 45 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Flüh, C., Mafael, V., Adamski, V., Synowitz, M., & Held‑Feindt, J. (2020). Dormancy and NKG2D system in brain metastases: Analysis of immunogenicity. International Journal of Molecular Medicine, 45, 298-314. https://doi.org/10.3892/ijmm.2019.4449
MLA
Flüh, C., Mafael, V., Adamski, V., Synowitz, M., Held‑Feindt, J."Dormancy and NKG2D system in brain metastases: Analysis of immunogenicity". International Journal of Molecular Medicine 45.2 (2020): 298-314.
Chicago
Flüh, C., Mafael, V., Adamski, V., Synowitz, M., Held‑Feindt, J."Dormancy and NKG2D system in brain metastases: Analysis of immunogenicity". International Journal of Molecular Medicine 45, no. 2 (2020): 298-314. https://doi.org/10.3892/ijmm.2019.4449