Histone H3K9 methylation is involved in temporomandibular joint osteoarthritis

  • Authors:
    • Mayumi Ukita
    • Kenji Matsushita
    • Masato Tamura
    • Taihiko Yamaguchi
  • View Affiliations

  • Published online on: December 27, 2019     https://doi.org/10.3892/ijmm.2019.4446
  • Pages: 607-614
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The morbidity of temporomandibular joint osteoarthritis (TMJOA) increases with age. Condylar articular cartilage degradation, which causes TMJOA, is known to be involved in articular chondrocyte metabolic imbalances in the temporomandibular joint (TMJ) and in other joints of the body. Epigenetic regulation, such as the chemical modification of DNA and histones, is implicated in cartilage homeostasis. However, few studies have been conducted on the epigenetic regulation of condylar articular cartilage degradation. The present study investigated the regulation of histone H3 lysine 9 (H3K9) methylation and its effects on the pathogenesis of degenerative TMJ cartilage disorders. The histone H3K9 methylation level was decreased in degenerated condylar articular cartilage in aged mice. Treatment with chaetocin (a selective H3K9 methylation inhibitor) reduced cell viability and promoted caspase‑3/7 activity in ATDC5 mouse chondroprogenitor cells. The inhibition of H3K9 methylation increased matrix metalloproteinase (Mmp)1 and Mmp13 mRNA expression in these cells. Furthermore, the expression levels of Sox9 and collagen α1(II) (Col2a1) mRNA, which are anabolic factors for chondrogenic differentiation, were also decreased by treatment with chaetocin, which is an inhibitor of histone methyltransferases. These results indicated that histone H3K9 methylation regulates chondrocyte homeostasis in terms of cell growth, apoptosis and gene expression, and highlighted a possible future therapy option for TMJOA.
View Figures
View References

Related Articles

Journal Cover

February 2020
Volume 45 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Ukita, M., Matsushita, K., Tamura, M., & Yamaguchi, T. (2020). Histone H3K9 methylation is involved in temporomandibular joint osteoarthritis. International Journal of Molecular Medicine, 45, 607-614. https://doi.org/10.3892/ijmm.2019.4446
MLA
Ukita, M., Matsushita, K., Tamura, M., Yamaguchi, T."Histone H3K9 methylation is involved in temporomandibular joint osteoarthritis". International Journal of Molecular Medicine 45.2 (2020): 607-614.
Chicago
Ukita, M., Matsushita, K., Tamura, M., Yamaguchi, T."Histone H3K9 methylation is involved in temporomandibular joint osteoarthritis". International Journal of Molecular Medicine 45, no. 2 (2020): 607-614. https://doi.org/10.3892/ijmm.2019.4446