Open Access

miR‑146a improves hepatic lipid and glucose metabolism by targeting MED1

  • Authors:
    • Kun Li
    • Bao Zhao
    • Diandian Wei
    • Wenrui Wang
    • Yixuan Cui
    • Lisheng Qian
    • Guodong Liu
  • View Affiliations

  • Published online on: December 27, 2019     https://doi.org/10.3892/ijmm.2019.4443
  • Pages: 543-555
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Non‑alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide. Increasing evidence has shown that microRNAs (miRNAs) play a vital role in the progression of NAFLD. The aim of the present study was to examine the expression level and roles of miR‑146a in fatty liver of high‑fat diet (HFD) and ob/ob mice and fatty acid‑treated hepatic cells using RT‑qPCR and western blot analysis. The results showed that the expression of miR‑146a was significantly decreased in the livers of high‑fat diet (HFD) and ob/ob mice and free fatty acid‑stimulated cells by RT‑qPCR. Overexpression of hepatic miR‑146a improved glucose and insulin tolerance as well as lipid accumulation in the liver by promoting the oxidative metabolism of fatty acids. In addition, the overexpression of miR‑146a increased the amount of mitochondria and promoted mitochondrial respiration in hepatocytes. Similarly, inhibition of miR‑146a expression levels significantly reduced mitochondrial numbers in AML12 cells as well as the expression of mitochondrial respiration related genes. Additionally, MED1 was a direct target of miR‑146a and restoring MED1 abolished the metabolic effects of miR‑146a on lipid metabolism and mitochondrial function. Therefore, results of the present study identified a novel function of miR‑146a in glucose and lipid metabolism in targeting MED1, suggesting that miR‑146a serves as a potential therapeutic target for metabolic syndrome disease.
View Figures
View References

Related Articles

Journal Cover

February 2020
Volume 45 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Li, K., Zhao, B., Wei, D., Wang, W., Cui, Y., Qian, L., & Liu, G. (2020). miR‑146a improves hepatic lipid and glucose metabolism by targeting MED1. International Journal of Molecular Medicine, 45, 543-555. https://doi.org/10.3892/ijmm.2019.4443
MLA
Li, K., Zhao, B., Wei, D., Wang, W., Cui, Y., Qian, L., Liu, G."miR‑146a improves hepatic lipid and glucose metabolism by targeting MED1". International Journal of Molecular Medicine 45.2 (2020): 543-555.
Chicago
Li, K., Zhao, B., Wei, D., Wang, W., Cui, Y., Qian, L., Liu, G."miR‑146a improves hepatic lipid and glucose metabolism by targeting MED1". International Journal of Molecular Medicine 45, no. 2 (2020): 543-555. https://doi.org/10.3892/ijmm.2019.4443