Open Access

Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats

  • Authors:
    • Wen-Zhan Tu
    • Si-Si Li
    • Xia Jiang
    • Xin-Ru Qian
    • Guan-Hu Yang
    • Peng-Peng Gu
    • Bin Lu
    • Song-He Jiang
  • View Affiliations

  • Published online on: March 13, 2018     https://doi.org/10.3892/ijmm.2018.3563
  • Pages: 3307-3315
  • Copyright: © Tu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Microglia, which comprise a sensor for pathological events in the central nervous system, may be triggered by nerve injury and transformed from a quiescent state into an activated state; ionised calcium binding adaptor molecule 1 (Iba1) is a sensitive marker associated with activated microglia. Accumulated evidence suggests that spinal activated microglia and the brain-derived neurotrophic factor (BDNF)-tyrosine kinase receptor B (TrkB) signalling pathway play major roles in the production and development of neuropathic pain. Electro-acupuncture (EA) has a positive effect on relieving chronic neuropathic pain; however, the underlying mechanisms remain unclear. To determine the significance of EA in the treatment of neuropathic pain mediated by activated microglia and the BDNF-TrkB signalling pathway in the spinal cord, the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) values were recorded to assess hyperalgesia and allodynia. In addition, the amount of activated microglia and BDNF were assessed via immunofluorescence. Iba1, BDNF and TrkB mRNA expression levels were examined using qPCR; the protein levels of BDNF, p-TrkB and TrkB in the spinal cord were analysed via western blotting. The present study demonstrated that EA treatment increased the MWT and TWL values. EA significantly inhibited the proportion of activated microglia and BDNF expression in the spinal cord after chronic constrictive injury (CCI). Furthermore, EA decreased the expression of BDNF and TrkB at both the mRNA and protein levels in the spinal cord of CCI rats. These findings suggest that the analgesic effect of EA may be achieved by inhibiting the activation of spinal microglia and subsequently blocking the BDNF-TrkB signalling pathway.
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June-2018
Volume 41 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos
Tu W, Li S, Jiang X, Qian X, Yang G, Gu P, et al: Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats. Int J Mol Med 41: 3307-3315, 2018
APA
Tu, W., Li, S., Jiang, X., Qian, X., Yang, G., Gu, P. ... Jiang, S. (2018). Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats. International Journal of Molecular Medicine, 41, 3307-3315. https://doi.org/10.3892/ijmm.2018.3563
MLA
Tu, W., Li, S., Jiang, X., Qian, X., Yang, G., Gu, P., Lu, B., Jiang, S."Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats". International Journal of Molecular Medicine 41.6 (2018): 3307-3315.
Chicago
Tu, W., Li, S., Jiang, X., Qian, X., Yang, G., Gu, P., Lu, B., Jiang, S."Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI rats". International Journal of Molecular Medicine 41, no. 6 (2018): 3307-3315. https://doi.org/10.3892/ijmm.2018.3563