Expression profiles of miRNAs in polarized macrophages

  • Authors:
    • Yingying Zhang
    • Mengying Zhang
    • Min Zhong
    • Qifeng Suo
    • Kun Lv
  • View Affiliations

  • Published online on: January 30, 2013     https://doi.org/10.3892/ijmm.2013.1260
  • Pages: 797-802
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Abstract

microRNAs (miRNAs) play a crucial role in tissue development and the pathology of various diseases. However, the effects and roles of miRNAs in macrophage polarization have yet to be investigated. In this study, we analyzed and compared the miRNA expression profiles of bone marrow-derived macrophages (BMDMs) with two distinct polarizing conditions (classical macrophage activation ‘M1’ and alternative activation ‘M2’) using miRNA microarray. In total, 109 miRNAs were differentially expressed between M1 and M2. The differential expression of selected miRNAs was validated by real-time qRT-PCR: miR-181a, miR-155-5p, miR-204-5p and miR-451 were upregulated (fold change >2, P<0.05) and miR-125-5p, miR-146a-3p, miR-143-3p and miR-145-5p were downregulated (fold change <-2, P<0.05) in M1 compared with M2. In conclusion, our study may be useful for exploring the precise roles of miRNAs in macrophage differentiation and polarized activation processes in the future.
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April 2013
Volume 31 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., & Lv, K. (2013). Expression profiles of miRNAs in polarized macrophages. International Journal of Molecular Medicine, 31, 797-802. https://doi.org/10.3892/ijmm.2013.1260
MLA
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., Lv, K."Expression profiles of miRNAs in polarized macrophages". International Journal of Molecular Medicine 31.4 (2013): 797-802.
Chicago
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., Lv, K."Expression profiles of miRNAs in polarized macrophages". International Journal of Molecular Medicine 31, no. 4 (2013): 797-802. https://doi.org/10.3892/ijmm.2013.1260