GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells

  • Authors:
    • Haiyan Qin
    • Guang Zhang
    • Lianbo Zhang
  • View Affiliations

  • Published online on: February 13, 2018     https://doi.org/10.3892/etm.2018.5863
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Polycomb group genes (PcG) encode chromatin modification proteins that are involved in the epigenetic regulation of cell differentiation, proliferation and the aging processes. The key subunit of the PcG complex, enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), has a central role in a variety of mechanisms, such as the formation of chromatin structure, gene expression regulation and DNA damage. In the present study, ultraviolet A (UVA) was used to radiate human dermal fibroblasts in order to construct a photo‑aged cell model. Subsequently, the cell viability assay, Hoechst staining, apoptosis detection using flow cytometry, senescence‑associated β‑galactosidase (SA‑β‑gal) staining and erythrocyte exclusion experiments were performed. GSK126, a histone methylation enzyme inhibitor of EZH2, was used as an experimental factor. Results suggested that GSK126 downregulated the mRNA expression levels of EZH2 and upregulated the mRNA expression levels of BMI‑1. Notably, GSK126 affected the transcription of various photoaging‑related genes and thus protected against photoaging induced by UVA radiation.

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Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Qin, H., Zhang, G., & Zhang, L. (1899). GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells. Experimental and Therapeutic Medicine, 0, 0-0. https://doi.org/10.3892/etm.2018.5863
MLA
Qin, H., Zhang, G., Zhang, L."GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells". Experimental and Therapeutic Medicine 0.0 (1899): 0-0.
Chicago
Qin, H., Zhang, G., Zhang, L."GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells". Experimental and Therapeutic Medicine 0, no. 0 (1899): 0-0. https://doi.org/10.3892/etm.2018.5863