Open Access

Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro

  • Authors:
    • Xiao‑Bo Tang
    • Pei‑Long Dong
    • Jian Wang
    • Hai‑Yang Zhou
    • Hai‑Xiang Zhang
    • Shan‑Zheng Wang
  • View Affiliations

  • Published online on: May 28, 2015     https://doi.org/10.3892/etm.2015.2528
  • Pages: 477-483
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study aimed to isolate rabbit adipose-derived stem cells (ADSCs) and explore the potential of platelet‑rich plasma (PRP) in the chondrogenic differentiation of ADSCs, thereby potentially providing a new approach for the repair and regeneration of cartilage injury. Rabbit ADSCs were isolated and characterized by induction towards adipogenic, osteogenic and chondrogenic lineages in vitro. The isolated ADSCs were also cultured with or without 10% PRP. Immunofluorescence staining, toluidine blue staining and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) were used to detect type II collagen (Col II) and aggrecan (AGC) expression. Col II immunofluorescence staining and toluidine blue staining indicated that following induction by autologous PRP, ADSCs manifested Col II and AGC expression. The expression of Col II and AGC mRNA was significantly upregulated in the PRP‑treated cells when compared with that in control cells. Autologous PRP produced by laboratory centrifugation was able to promote the chondrogenic differentiation of rabbit ADSCs in vitro.
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August 2015
Volume 10 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Tang, X., Dong, P., Wang, J., Zhou, H., Zhang, H., & Wang, S. (2015). Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro. Experimental and Therapeutic Medicine, 10, 477-483. https://doi.org/10.3892/etm.2015.2528
MLA
Tang, X., Dong, P., Wang, J., Zhou, H., Zhang, H., Wang, S."Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro". Experimental and Therapeutic Medicine 10.2 (2015): 477-483.
Chicago
Tang, X., Dong, P., Wang, J., Zhou, H., Zhang, H., Wang, S."Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro". Experimental and Therapeutic Medicine 10, no. 2 (2015): 477-483. https://doi.org/10.3892/etm.2015.2528