Open Access

Anti‑angiogenic and anti‑proliferative effects of Benja‑ummarit extract in rats with hepatocellular carcinoma

  • Authors:
    • Nattpawit Kaewnoonual
    • Arunporn Itharat
    • Suriya Pongsawat
    • Cheng Nilbu‑Nga
    • Vichununt Kerdput
    • Wisuit Pradidarcheep
  • View Affiliations

  • Published online on: January 16, 2020     https://doi.org/10.3892/br.2020.1272
  • Pages: 109-120
  • Copyright: © Kaewnoonual et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The herbal extract Benja‑ummarit (BU) is a traditional Thai medicine with a putative cancer‑suppressing effect. However, this effect has only been tested in vitro in human hepatocarcinoma cell lines. The present study determined the efficacy of a BU extract to treat hepatocellular carcinoma (HCC) in rats in vivo and established its anti‑angiogenic and anti‑proliferative properties. The BU extract was prepared in 95% ethanol and its composition determined using liquid chromatography‑mass spectrometry. HCC was induced in Wistar rats by an injection of diethylnitrosamine (DEN), followed 2 weeks later by injections of thioacetamide (TAA) thrice weekly for 4 weeks. Following 2 months, the DEN‑TAA‑treated rats were divided into 6 groups that were treated orally for another 2 months with: i) No treatment; ii) vehicle; iii) 30 mg/kg sorafenib (SF); iv) 1 mg/kg BU; v) 10 mg/kg BU; or vi) 50 mg/kg BU. Liver samples were collected for gross morphological, histological, reverse transcription‑quantitative PCR and western blot analyses, and serum samples were collected for liver function tests. The size and number of the cancer nodules were reduced ~10‑fold in BU‑treated HCC groups and ~14‑fold in the SF‑treated group compared with the HCC group. Furthermore, the serum parameters of liver damage were lower in BU‑compared with SF‑treated rats. These results indicate that while each of these formulations strongly reduce HCC expansion, BU extract results in less liver damage. Vascular endothelial growth factor expression was reduced significantly in the BU‑and SF‑treated HCC groups compared with the HCC group (P<0.05). BU extract antagonizes HCC growth in vivo potently through inhibiting tumor angiogenesis. BU, therefore, qualifies as a promising medical herb requiring further evaluation as a treatment of HCC.
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March 2020
Volume 12 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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APA
Kaewnoonual, N., Itharat, A., Pongsawat, S., Nilbu‑Nga, C., Kerdput, V., & Pradidarcheep, W. (2020). Anti‑angiogenic and anti‑proliferative effects of Benja‑ummarit extract in rats with hepatocellular carcinoma. Biomedical Reports, 12, 109-120. https://doi.org/10.3892/br.2020.1272
MLA
Kaewnoonual, N., Itharat, A., Pongsawat, S., Nilbu‑Nga, C., Kerdput, V., Pradidarcheep, W."Anti‑angiogenic and anti‑proliferative effects of Benja‑ummarit extract in rats with hepatocellular carcinoma". Biomedical Reports 12.3 (2020): 109-120.
Chicago
Kaewnoonual, N., Itharat, A., Pongsawat, S., Nilbu‑Nga, C., Kerdput, V., Pradidarcheep, W."Anti‑angiogenic and anti‑proliferative effects of Benja‑ummarit extract in rats with hepatocellular carcinoma". Biomedical Reports 12, no. 3 (2020): 109-120. https://doi.org/10.3892/br.2020.1272