Open Access

Expression profiling of genes in rheumatoid fibroblast‑like synoviocytes regulated by tumor necrosis factor‑like ligand 1A using cDNA microarray analysis

  • Authors:
    • Koji Fukuda
    • Yasushi Miura
    • Toshihisa Maeda
    • Shinya Hayashi
    • Ryosuke Kuroda
  • View Affiliations

  • Published online on: May 22, 2019     https://doi.org/10.3892/br.2019.1216
  • Pages: 11-18
  • Copyright: © Fukuda et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Rheumatoid arthritis (RA) is an autoimmune disease that causes chronic inflammation in synovial tissues. Hyperplasia of synovial tissue leads to the formation of pannus, which invades joint cartilage and bone resulting in joint destruction. Tumor necrosis factor‑like ligand 1A (TL1A), a member of the tumor necrosis factor superfamily (TNFSF15), contributes to the pathogenesis of autoimmune diseases, including RA. In the present study, a cDNA microarray was used to search for genes whose expression in rheumatoid fibroblast‑like synoviocytes (RA‑FLS) were regulated by TL1A. Four individual lines of primary cultured RA‑FLS were incubated either with recombinant human TL1A protein or phosphate‑buffered saline, as an unstimulated control, for 12 h. Gene expression was then detected through the microarray assay. The results revealed the expression profiles of genes in RA‑FLS regulated by TL1A. The present study also demonstrated the functions of those genes whose expression in RA‑FLS was regulated by TL1A. Among the genes in this profile, the present study focused on the following genes: Spectrin repeat‑containing nuclear envelope 1, Fc receptor‑like 2, PYD (pyrin domain)‑containing 1, cell division cycle 45 homolog, signal transducer and activator of transcription 5B, and interferon regulatory factor 4. These genes may affect the pathogenesis of RA, including proliferation, regulation of B cells and T cells, inflammation, and cytokine processing. The present study revealed for the first time, to the best of our knowledge, the expression profile of genes in RA‑FLS regulated by TL1A. The data indicate that TL1A may regulate the gene expression of various key molecules in RA‑FLS, thus affecting the pathogenesis of RA. Further investigations of the genes detected in the current profiles may provide a deeper understanding of the pathogenesis and a novel target for the treatment of RA.

Related Articles

Journal Cover

July 2019
Volume 11 Issue 1

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Fukuda, K., Miura, Y., Maeda, T., Hayashi, S., & Kuroda, R. (2019). Expression profiling of genes in rheumatoid fibroblast‑like synoviocytes regulated by tumor necrosis factor‑like ligand 1A using cDNA microarray analysis. Biomedical Reports, 11, 11-18. https://doi.org/10.3892/br.2019.1216
MLA
Fukuda, K., Miura, Y., Maeda, T., Hayashi, S., Kuroda, R."Expression profiling of genes in rheumatoid fibroblast‑like synoviocytes regulated by tumor necrosis factor‑like ligand 1A using cDNA microarray analysis". Biomedical Reports 11.1 (2019): 11-18.
Chicago
Fukuda, K., Miura, Y., Maeda, T., Hayashi, S., Kuroda, R."Expression profiling of genes in rheumatoid fibroblast‑like synoviocytes regulated by tumor necrosis factor‑like ligand 1A using cDNA microarray analysis". Biomedical Reports 11, no. 1 (2019): 11-18. https://doi.org/10.3892/br.2019.1216