Quantification of preeclampsia-related microRNAs in maternal serum

  • Authors:
    • Qian Li
    • Anxiong Long
    • Liansheng Jiang
    • Leiming Cai
    • Li Xie
    • Ji'an Gu
    • Xiong Chen
    • Longyi Tan
  • View Affiliations

  • Published online on: September 30, 2015     https://doi.org/10.3892/br.2015.524
  • Pages: 792-796
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To identify the specific serum preeclampsia (PE)-related biomarkers, 10 microRNAs (miRNAs) were selected based on their reported aberrant (4 upregulated and 6 downregulated) expression in PE placenta. A total of 1,035 pregnant patients were enrolled. Finally, 32 pregnancies with PE and 32 healthy pregnancies were incorporated in the study. The expression of these 10 miRNAs in the different trimesters was determined by SYBR‑Green reverse transcription‑quantitative polymerase chain reaction. Compared with that in the healthy controls, the expression levels of miR‑152, miR‑183 and miR‑210 in PE serum were higher in the second and third trimester, whereas the expression of miR‑182 was only higher in the third trimester. The expression levels of 6 miRNAs (miR‑1, miR‑328, miR‑363, miR‑377, miR‑500 and miR‑584) that were downregulated in PE placenta showed no significant differences between pregnancies complicated by PE and healthy pregnancies throughout the 3 trimesters. Areas under the receiver operating characteristic [standard error (SE)] during the 20‑24th gestational week for predicting PE were miR‑152: 0.94 (SE, 0.026), miR‑183: 0.97 (SE, 0.031) and miR‑210: 0.93 (SE, 0.018). In conclusion, the expression levels of serum miR‑152, miR‑183 and miR‑210 were elevated since the second trimester in pregnancies complicated with PE, indicating their potentials as serum biomarkers for forecasting PE.

References

1 

Duley L: The global impact of pre-eclampsia and eclampsia. Semin Perinatol. 33:130–137. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Redman CW and Sargent IL: Preeclampsia and the systemic inflammatory response. Semin Nephrol. 24:565–570. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Pineles BL, Romero R, Montenegro D, Tarca AL, Han YM, Kim YM, Draghici S, Espinoza J, Kusanovic JP, Mittal P, et al: Distinct subsets of microRNAs are expressed differentially in the human placentas of patients with preeclampsia. Am J Obstet Gynecol. 196:261.e1–6. 2007. View Article : Google Scholar

4 

Zhu XM, Han T, Sargent IL, Yin GW and Yao YQ: Differential expression profile of microRNAs in human placentas from preeclamptic pregnancies vs normal pregnancies. Am J Obstet Gynecol. 200:661.e1–7. 2009. View Article : Google Scholar

5 

Hu Y, Li P, Hao S, Liu L, Zhao J and Hou Y: Differential expression of microRNAs in the placentae of Chinese patients with severe pre-eclampsia. Clin Chem Lab Med. 47:923–929. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Enquobahrie DA, Abetew DF, Sorensen TK, Willoughby D, Chidambaram K and Williams MA: Placental microRNA expression in pregnancies complicated by preeclampsia. Am J Obstet Gynecol. 204:178. e12–21. 2011. View Article : Google Scholar

7 

Ji L, Brkić J, Liu M, Fu G, Peng C and Wang YL: Placental trophoblast cell differentiation: Physiological regulation and pathological relevance to preeclampsia. Mol Aspects Med. 34:981–1023. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O'Briant KC, Allen A, et al: Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA. 105:10513–10518. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Noack F, Ribbat-Idel J, Thorns C, Chiriac A, Axt-Fliedner R, Diedrich K and Feller AC: miRNA expression profiling in formalin-fixed and paraffin-embedded placental tissue samples from pregnancies with severe preeclampsia. J Perinat Med. 39:267–271. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Betoni JS, Derr K, Pahl MC, Rogers L, Muller CL, Packard E, Carey DJ, Kuivaniemi H and Tromp G: MicroRNA analysis in placentas from patients with preeclampsia: Comparison of new and published results. Hypertens Pregnancy. 32:321–339. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Hromadnikova I, Kotlabova K, Ondrackova M, Kestlerova A, Novotna V, Hympanova L, Doucha J and Krofta L: Circulating C19MC microRNAs in preeclampsia, gestational hypertension, and fetal growth restriction. Mediators Inflamm. 2013:1860412013. View Article : Google Scholar : PubMed/NCBI

12 

Gunel T, Zeybek YG, Akçakaya P, Kalelioğlu I, Benian A, Ermis H and Aydınlı K: Serum microRNA expression in pregnancies with preeclampsia. Genet Mol Res. 10:4034–4040. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Hromadnikova I, Kotlabova K, Doucha J, Dlouha K and Krofta L: Absolute and relative quantification of placenta-specific micrornas in maternal circulation with placental insufficiency-related complications. J Mol Diagn. 14:160–167. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Yang Q, Lu J, Wang S, Li H, Ge Q and Lu Z: Application of next-generation sequencing technology to profile the circulating microRNAs in the serum of preeclampsia versus normal pregnant women. Clin Chim Acta. 412:2167–2173. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Zhang Y, Fei M, Xue G, Zhou Q, Jia Y, Li L, Xin H and Sun S: Elevated levels of hypoxia-inducible microRNA-210 in pre-eclampsia: New insights into molecular mechanisms for the disease. J Cell Mol Med. 16:249–259. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Xu P, Zhao Y, Liu M, Wang Y, Wang H, Li YX, Zhu X, Yao Y, Wang H, Qiao J, et al: Variations of microRNAs in human placentas and plasma from preeclamptic pregnancy. Hypertension. 63:1276–1284. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Ishibashi O, Ohkuchi A, Ali MM, Kurashina R, Luo SS, Ishikawa T, Takizawa T, Hirashima C, Takahashi K, Migita M, et al: Hydroxysteroid (17-β) dehydrogenase 1 is dysregulated by miR-210 and miR-518c that are aberrantly expressed in preeclamptic placentas: A novel marker for predicting preeclampsia. Hypertension. 59:265–273. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Tan Z, Randall G, Fan J, Camoretti-Mercado B, Brockman-Schneider R, Pan L, Solway J, Gern JE, Lemanske RF, Nicolae D, et al: Allele-specific targeting of microRNAs to HLA-G and risk of asthma. Am J Hum Genet. 81:829–834. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Yie SM, Li LH, Li YM and Librach C: HLA-G protein concentrations in maternal serum and placental tissue are decreased in preeclampsia. Am J Obstet Gynecol. 191:525–529. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Heazell AE and Crocker IP: Live and let die-regulation of villous trophoblast apoptosis in normal and abnormal pregnancies. Placenta. 29:772–783. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Smith GC and Wear H: The perinatal implications of angiogenic factors. Curr Opin Obstet Gynecol. 21:111–116. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Zhang QH, Sun HM, Zheng RZ, Li YC, Zhang Q, Cheng P, Tang ZH and Huang F: Meta-analysis of microRNA-183 family expression in human cancer studies comparing cancer tissues with noncancerous tissues. Gene. 527:26–32. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Shi XY, Gu L, Chen J, Guo XR and Shi YL: Downregulation of miR-183 inhibits apoptosis and enhances the invasive potential of endometrial stromal cells in endometriosis. Int J Mol Med. 33:59–67. 2014.PubMed/NCBI

24 

Wu L, Zhou H, Lin H, Qi J, Zhu C, Gao Z and Wang H: Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies. Reproduction. 143:389–397. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

Journal Cover

November 2015
Volume 3 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Li, Q., Long, A., Jiang, L., Cai, L., Xie, L., Gu, J. ... Tan, L. (2015). Quantification of preeclampsia-related microRNAs in maternal serum. Biomedical Reports, 3, 792-796. https://doi.org/10.3892/br.2015.524
MLA
Li, Q., Long, A., Jiang, L., Cai, L., Xie, L., Gu, J., Chen, X., Tan, L."Quantification of preeclampsia-related microRNAs in maternal serum". Biomedical Reports 3.6 (2015): 792-796.
Chicago
Li, Q., Long, A., Jiang, L., Cai, L., Xie, L., Gu, J., Chen, X., Tan, L."Quantification of preeclampsia-related microRNAs in maternal serum". Biomedical Reports 3, no. 6 (2015): 792-796. https://doi.org/10.3892/br.2015.524